| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Food, beverage, biological samples (e.g. serum, plasma, etc). |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of total carbohydrate in food, beverage, biological samples (e.g. serum, plasma, etc). The assay uses OD490nm for signal readout. Compatible sample input includes Food, beverage, biological samples (e.g. serum, plasma, etc).. Typical stated assay timing is 15 min.
Key elements and design rationale
- Readout format: OD490nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Food, beverage, biological samples (e.g. serum, plasma, etc)., which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.02 mM for interpreting low-signal samples.
- Feature emphasis: Non-radioactive assay.
Additional feature notes highlight Sensitive and accurate. Linear detection range in 96-well plate: 0.02 to 5 mM glucose equivalents for colorimetric assays; Fast and convenient. The procedure involves 15 minutes of incubation time and minimal sample preparation. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of total carbohydrate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
CARBOHYDRATESare made up of carbon, hydrogen, and oxygen. A carbohydrate may exist in its simplest form, a monosaccharide, or as larger polymers called polysaccharides (e.g. starch, glycogen, and cellulose). Carbohydrates are essential constituents of living things, and in the form of glucose, serve as the main source of energy for metabolism. They are also commonly found in many fruits and vegetables as simple or complex sugars.BioAssay Systems’ QuantiChrom™ Total Carbohydrate Assay Kit first hydrolyzes polysaccharides to monosaccharides, which are then converted to furfural compounds that react with the Detection Reagent. The color intensity of the chromagen, measured at 490 nm, is directly proportional to the total carbohydrate concentration (glucose equivalents) in the sample.
Detection method
Colorimetric (OD 490 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.02 mM.
Procedures and timing
Stated procedure or timing information: 15 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
- Matched standards, blanks, and replicate wells are typically used to improve interpretability across batches and sample matrices.
Common research applications
- Quantify total carbohydrate in food, beverage, biological samples (serum by OD490 nm readout.
- Compare treatment or phenotype groups using matched food, beverage, biological samples (serum handling.
- Monitor time-course or pre/post changes in food, beverage, biological samples (serum across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the Detection Reagent react with all carbohydrates? If so, is it to the same degree?
The assay reacts with all carbohydrates (monosaccharides, disaccharides, etc.). The absorbance of equimolar sugars will differ and therefore it is important that the concentration of carbohydrates in a sample is arbitrarily expressed as the equivalents of one sugar (D-glucose in the case of TCRB-100).
Can the entire assay be performed in a microplate?
The assay was designed to be performed in microcentrifuge tubes and then transferred to a microplate for reading. However, as long as the material of the microplate is not sensitive to sulfuric acid (e.g. polystyrene), the assay should be able to be done from start to finish in a microplate. It is recommended to test the resilience of plastics to sulfuric acid prior to performing the assay by adding 100 μL of concentrated sulfuric acid (95%) and heating at 90°C for 5 minutes.
During the heating step, cover the plate with a sealing film to prevent loss of solution. Upon the addition of the Detection Reagent to each well, it is necessary to pipette up and down multiple times to mix well. After the 10 minute incubation with the Detection Reagent, transfer 100 μL from each well to separate wells in a clear, flat bottom 96-well plate.
If my sample has a mixture of carbohydrates, how accurate are the results?
Since the absorbance of the colored product varies in response to different sugars, this assay may over- or underestimate carbohydrate content in mixtures or biological samples.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
