QuantiChrom™ Total Protein Assay Kit

Overview

For quantitative determination of total protein. The assay uses OD600nm (Pyrogallol Red) for signal readout. Compatible sample input includes Urine, cerebrospinal fluid, etc. Typical stated assay timing is 10 min.

Key elements and design rationale

• Readout format: OD600nm (Pyrogallol Red) supports plate-based signal acquisition and consistent comparison across matched samples.
• Sample compatibility: The stated sample scope includes Urine, cerebrospinal fluid, etc, which is useful when aligning matrix type with calibration and control design.
• Analytical range context: The supplied specifications include a stated detection limit of 5 mg/dL for interpreting low-signal samples.
• Feature emphasis: Fast and sensitive. The assay takes 10 min. Linear detection range of 5 – 200 mg/dL protein.

Additional feature notes highlight Robust and Reliable. Substances in urine have minimal interference (< 2%); Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.

Biological background

This product is centered on measurement of total protein within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.

More details

Total protein concentration can be determined by several methods. However, protein dye-binding procedures are simple, reliable, rapid, and readily automated and are becoming increasingly popular in assessing renal and blood-brain barrier functions. BioAssay Systems Total Protein Assay Kit is based on an improved pyrogallol red-molybdate protein dye-binding assay. The color intensity at 600nm is directly proportional to the total protein concentration in the sample. This single reagent can be used for the quantitative determination of total protein in urine (proteinuria) and cerebrospinal fluid (blood-brain barrier).

Detection method

Colorimetric (OD 600 nm).

Detection limit and analytical sensitivity

Reported detection limit: 5 mg/dL.

Procedures and timing

Stated procedure or timing information: 10 min.

Research relevance and current trends

• Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
• The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
• Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.

Common research applications

• Quantify total protein in urine, cerebrospinal fluid by OD600 nm (Pyrogallol Red) readout.
• Compare treatment or phenotype groups using matched urine, cerebrospinal fluid handling.
• Monitor time-course or pre/post changes in urine, cerebrospinal fluid across study conditions.

Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.

Notes for experimental interpretation

• Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
• Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.

For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.