| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Sample type(s) | Biological |
| Detection method | |
| Assay time | |
| Storage | |
| Shelf life | |
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| Catalog no. (Mfr.) | |
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Overview
Stabilized 4-methylumbelliferyl phosphate (MUP) liquid substrate reagent for detecting alkaline phosphatase ALP, e.g. in ELISA. This single reagent produces a highly fluorescent product (360/450 nm) when dephosphorylated by ALP. The. The assay uses FL360/450nm for signal readout. Compatible sample input includes Biological. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: FL360/450nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 30 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Stable single reagent.
Additional feature notes highlight Highly sensitive fluorescence detection; Can be visualized under a UV light. Available format information for this listing includes 600 Tests.
Biological background
This product is centered on measurement of alp detection within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
QuantiFluo™ ALP Reagent is a stable 4-methylumbelliferyl phosphate (MUP) liquid substrate reagent formulated for detecting alkaline phosphatase ALP, e.g. in ELISA. This single reagent produces a highly fluorescent product (λex/em = 360/450 nm) when dephosphorylated by ALP. The fluorescent product can also be visualized under a UV light.
Detection method
Fluorescent (FL 360/450 nm).
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
- Matched standards, blanks, and replicate wells are typically used to improve interpretability across batches and sample matrices.
Common research applications
- Prepare control-treated samples for paired use with compatible assay-kit plates.
- Benchmark assay response against defined control conditions in replicate wells.
- Optimize reagent concentration and incubation windows for plate-based comparisons.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
