| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, Plasma, urine, and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of ammonia and ammonium in biological samples. The assay uses FL415/475 nm for signal readout. Compatible sample input includes Serum, Plasma, urine, and other biological samples. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: FL415/475 nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, Plasma, urine, and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 5.3 µM for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Linear detection range of 0.005 – 2mM ammonia.
Additional feature notes highlight Convenient and high-throughput. A single component reagent. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of ammonia within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3are very desirable. BioAssay Systems’ updated ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 415/475nm) is proportional to the ammonia concentration in the sample.
Detection method
Fluorescent (FL 415/475 nm).
Detection limit and analytical sensitivity
Reported detection limit: 5.3 µM.
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify ammonia in serum, Plasma, urine by FL415/475 nm readout.
- Compare treatment or phenotype groups using matched serum, Plasma, urine handling.
- Monitor time-course or pre/post changes in serum, Plasma, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
