| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, cell or tissue lysate, and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of ACE-1 activity determination in biological samples. Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Sensitive and accurate. The assay uses FL360/485nm for signal readout. Compatible sample input includes Serum, cell or tissue lysate, and other biological samples. Typical stated assay timing is 45 min.
Key elements and design rationale
- Readout format: FL360/485nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, cell or tissue lysate, and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.01 U/L for interpreting low-signal samples.
- Feature emphasis: Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
Additional feature notes highlight Sensitive and accurate. Linear detection range 0.01 – 5 U/L ACE-1 in a 96-well plate assay; High-throughput. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of angiotensin-converting enzyme 1 within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
ANGIOTENSIN-CONVERTING ENZYME 1, ACE-1 (EC 3.4.15.1)is a critical enzyme in the renin-angiotensin-aldosterone system (RAAS), which regulates blood pressure, fluid, and electrolyte balance in the body. Found primarily in the lung endothelium and kidney epithelial cells, ACE-1 hydrolyzes angiotensin I, cleaving off the His-Leu dipeptide to produce angiotensin II. Its role in the RAAS system makes it a major target for hypertension and cardiovascular treatments.BioAssay Systems’ QACE1-100 Kit provides a convenient fluorometric method to measure ACE-1 activity in biological samples. In this assay, ACE-1 hydrolyzes the synthetic substrate, releasing the product. Then, o-phthalaldehyde (OPA) fluorescently labels the product for quantification. The increase in fluorescence at λex/em= 360/485 nm is directly proportional to enzyme activity.
Detection method
Fluorescent (FL 360/485 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.01 U/L.
Procedures and timing
Stated procedure or timing information: 45 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify angiotensin-converting enzyme 1 in serum, cell or tissue lysate by FL360/485 nm readout.
- Compare treatment or phenotype groups using matched serum, cell or tissue lysate handling.
- Monitor time-course or pre/post changes in serum, cell or tissue lysate across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
My Standard component appears to contain precipitate upon thawing to room temperature. Has this been observed before?
Yes, this is actually quite common. Vortex the Standard and the solid should quickly dissolve. The Standard has undergone many freeze/thaw cycles with this same observation and results are always consistent.
Would it be possible to combine the Working Reagent and Detection Reagent into one reagent and read for 45 minutes instead of separating the steps?
No, the mechanism behind this reaction includes two parts. The first step involves the addition of Working Reagent with Substrate with the enzyme catalyzing the reaction. The second step uses the Detection Reagent to stop the reaction and detect the product. Both steps are necessary and must be carried out separately.
Does this kit measure angiotensin-converting enzyme 2 (ACE2) in addition to angiotensin-converting enzyme 1 (ACE1)?
This kit is specific to ACE1 due to the differences in substrate specificity between ACE1 and ACE2.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
