| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Sample type(s) | Compounds that affect chymotrypsin protease activity. |
| Detection method | |
| Storage | |
| Shelf life | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
For evaluation of drugs and screening of potential inhibitors to chymotrypsin proteases. Safe. Non-radioactive assay. Homogeneous and convenient. Homogenous “Mix-incubate-measure” type assay. No wash and reagent transfer steps are. The assay uses FL485/530 nm for signal readout. Compatible sample input includes Compounds that affect chymotrypsin protease activity.. Typical stated assay timing is 45 min.
Key elements and design rationale
- Readout format: FL485/530 nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Compounds that affect chymotrypsin protease activity., which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 45 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Safe. Non-radioactive assay.
Additional feature notes highlight Homogeneous and convenient. Homogenous “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved; Robust and High-throughput. Can be readily automated to assay thousands of samples per day. Robust assay with a Z’ factor of >0.8. Available format information for this listing includes 100 Tests in 96-well plate (Custom bulk sizes available upon request).
Biological background
This product is centered on measurement of chymotrypsin inhibitor within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
CHYMOTRYPSIN(EC 3.4.21.1) is a digestive, serine protease that hydrolyzes dietary proteins in many eukaryotic and prokaryotic organisms. Chymotrypsin predominantly cleaves peptide chains at the carboxyl side of the aromatic phenylalanine, tryptophan, and tyrosine amino acids. BioAssay System’s QuantiFluo™ Chymotrypsin Inhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled synthetic substrate. The fluorescein label is highly quenched. Upon digestion by chymotrypsin present in the sample, the substrate is cleaved into smaller peptides, which abolishes the quenching of the fluorescence label. The fluorescence or fluorescence polarization (FP) of the FITC-labeled fragments is measured at λex/em= 485/530 nm. Inhibition is determined by the decrease in fluorescence.
Detection method
Fluorescent (FL 485/530 nm).
Procedures and timing
Stated procedure or timing information: 45 min.
Screening services
BioAssay Systems offers comprehensive Chymotrypsin lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us for more details at Service .
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify chymotrypsin inhibitor in compounds that affect chymotrypsin protease by FL485/530 nm readout.
- Compare treatment or phenotype groups using matched compounds that affect chymotrypsin protease handling.
- Monitor time-course or pre/post changes in compounds that affect chymotrypsin protease across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Is chymotrypsin enzyme or inhibitor included in the kit?
The customer must provide their own enzyme and inhibitor. We used purified chymotrypsin (MedChemExpress, cat# HY108910) and trypsin soybean inhibitor (Cayman Chemical, cat# 14502)
Can the substrate be refrozen after use?
While the substrate can handle a few freeze-thaw cycles, if the customer plans to run many tests, it would be best to aliquot the substrate into light-protected tubes and thaw as needed.
Does chymotrypsin solution need to be prepared fresh for each assay run? I know that, after some time, proteases like chymotrypsin may begin to digest themselves.
To prevent autodigestion, pure chymotrypsin solutions can be stored long term at -80°C under acidic conditions (1 mM HCl, pH 3.0). 2 mM CaCl2 can be added to stabilize the stock solution.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
