| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, Plasma |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of diamine oxidase and evaluation of drug modulators. The assay uses FL530/585nm for signal readout. Compatible sample input includes Serum, Plasma. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, Plasma, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.5 U/L for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Use of 10 µL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed; High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of diamine oxidase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
DIAMINE OXIDASE (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.BioAssay Systems non-radioactive, fluorimetric DAO assay is based on the oxidation of putrescine to pyrroline plus NH3and H2O2. The generated H2O2is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λex/em = 530/585 nm is directly proportional to the enzyme activity.• Enzyme-Activity-Assays.htm” target=”_blank”>Other Enzyme Activity Assay Kits• Metabolism.htm” target=”_blank”>Other Metabolism Assay Kits
Detection method
Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.5 U/L.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify diamine oxidase in serum, Plasma by FL530/585 nm readout.
- Compare treatment or phenotype groups using matched serum, Plasma handling.
- Monitor time-course or pre/post changes in serum, Plasma across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
How do I store the kit?
This kit is shipped at room temperature. Upon receiving, please keep the kit in the freezer (-20 to -10°C) for long term stability.
What samples have you tested?
Serum samples were tested. Since DAO is copper dependent, EDTA should be avoided in sample preparation.
What is the benefit of using an internal standard?
Serum and plasma samples greatly influence the HRP reaction. In order to measure an appropriate RFU for H2O2 in a serum or plasma matrix, an internal standard is required.
Will the assay kit work in a 384 well plate, cuvette, etc?
Yes, the assay can be used in any standard black plate or cuvette. Simply adjust the total reaction volume to your container. For 384 well use 20-90 μL, for a cuvette use 1 mL (some narrow cuvettes can hold 200-500 μL).
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay and used within 1 hour.
I don’t have the correct wavelength filter, will the assay still work?
Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
