| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Sample type(s) | Biological, e.g. cells, tissue, proteins, peptide |
| Detection limit | |
| Detection method | |
| Assay time | |
| Storage | |
| Shelf life | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
For quantitative determination of total protein. The assay uses FL360/450nm for signal readout. Compatible sample input includes Biological, e.g. cells, tissue, proteins, peptide. Typical stated assay timing is 5 min.
Key elements and design rationale
- Readout format: FL360/450nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological, e.g. cells, tissue, proteins, peptide, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 1.3 µg/mL for interpreting low-signal samples.
- Feature emphasis: Simple and fast. A single reagent and assay completed within 5 min.
Additional feature notes highlight Sensitive and wide detection range. Linear detection range of 1.3 – 1000 µg/mL BSA; Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 200 Tests.
Biological background
This product is centered on measurement of protein within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
The protein or peptide is known as the “building blocks of life” and is one of the most important macromolecules in life science. Protein determination is a very common practice. Simple, direct and automation-ready procedures for measuring protein or peptide concentration are very desirable. BioAssay Systems QuantiFluo™ protein assay kit is based on an improved o-phthalaldehyde method. This reagent reacts with primary amines in protein or peptide and forms a blue fluorescent product, allowing detection of nanograms of proteins. The fluorescence intensity (λex/em = 360/450nm) is proportional to the protein concentration in the sample.
Detection method
Fluorescent (FL 360/450 nm).
Detection limit and analytical sensitivity
Reported detection limit: 1.3 µg/mL.
Procedures and timing
Stated procedure or timing information: 5 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify protein in biological, e.g. cells, tissue by FL360/450 nm readout.
- Compare treatment or phenotype groups using matched biological, e.g. cells, tissue handling.
- Monitor time-course or pre/post changes in biological, e.g. cells, tissue across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
