| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological Samples (e.g. Urine and serum) |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of urokinase activity determination in biological samples. The assay uses FL380/450nm for signal readout. Compatible sample input includes Biological Samples (e.g. Urine and serum). Typical stated assay timing is 15min.
Key elements and design rationale
- Readout format: FL380/450nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological Samples (e.g. Urine and serum), which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.04 U/L for interpreting low-signal samples.
- Feature emphasis: Safe. Non-radioactive assay.
Additional feature notes highlight Fast. Assay is completed within a 15 minute reaction time; Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of urokinase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
UROKINASE PLASMINOGEN ACTIVATOR (urokinase, uPA)is a key serine protease involved in the degradation of the extracellular matrix that catalyzes the conversion of plasminogen to active plasmin. It acts as a thrombolytic agent to break up blood clots and when over-expressed, has been reported to influence the growth of certain malignant tumors (breast, prostate, etc). BioAssay Systems’ DUKN-100 Kit provides a convenient fluorimetric method to measure urokinase activity in biological samples. In this assay, the fluorimetric substrate reacts with urokinase so that the increase in fluorescence at λex/em = 380/450 nm is directly proportional to enzyme activity.
Detection method
Fluorescent (FL 380/450 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.04 U/L.
Procedures and timing
Stated procedure or timing information: 15min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify urokinase in urine and serum by FL380/450 nm readout.
- Compare treatment or phenotype groups using matched urine and serum handling.
- Monitor time-course or pre/post changes in urine and serum across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the kit come with Urokinase enzyme?
No, the user needs to provide their own purified enzyme.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
