| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Sample type(s) | Compounds that affect urokinase activity |
| Detection limit | |
| Detection method | |
| Assay time | |
| Storage | |
| Shelf life | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
For evaluation of drugs and screening potential inhibitors of urokinase. The assay uses FL380/450nm for signal readout. Compatible sample input includes Compounds that affect urokinase activity. Typical stated assay timing is 30min.
Key elements and design rationale
- Readout format: FL380/450nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Compounds that affect urokinase activity, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.04 U/L for interpreting low-signal samples.
- Feature emphasis: Safe. Non-radioactive assay.
Additional feature notes highlight Fast. Assay is completed within a 30 minute reaction time; Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests (Custom bulk sizes available upon request).
Biological background
This product is centered on measurement of urokinase inhibitor screening within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
UROKINASE PLASMINOGEN ACTIVATOR (urokinase, uPA)is a key serine protease involved in the degradation of the extracellular matrix that catalyzes the conversion of plasminogen to active plasmin. It acts as a thrombolytic agent to break up blood clots and when over-expressed, has been reported to influence the growth of certain malignant tumors (breast, prostate, etc.). BioAssay Systems’ DUKI-100 Kit provides a convenient fluorimetric means to screen for potential urokinase inhibitors. In this assay, the fluorimetric substrate reacts with urokinase and an inhibitor will decrease the fluorescence at λex/em = 380/450 nm.
Detection method
Fluorescent (FL 380/450 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.04 U/L.
Procedures and timing
Stated procedure or timing information: 30min.
Screening services
BioAssay Systems offers comprehensive Urokinase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service .
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify urokinase inhibitor screening in compounds that affect urokinase activity by FL380/450 nm readout.
- Compare treatment or phenotype groups using matched compounds that affect urokinase activity handling.
- Monitor time-course or pre/post changes in compounds that affect urokinase activity across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the kit come with Urokinase enzyme?
No, the user needs to provide their own purified enzyme.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
