Overview
For rapid, direct determination of pyruvate concentrations in food and beverage samples as well as biological samples. The assay uses Visual for signal readout. Compatible sample input includes Wine, beer, biological samples, etc. Typical stated assay timing is Approximately 15 min.
Key elements and design rationale
- Readout format: Visual supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Wine, beer, biological samples, etc, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of Approximately 15 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Fast and sensitive. Use of 50, 100, or 400 µL sample. Semi-quantitative measurement between 0-131 mg/L (undiluted) Pyruvate.
Additional feature notes highlight Convenient. No expensive plate or cuvette readers needed; Sample treatment and assay can be performed in under 15 minutes. Available format information for this listing includes 10 Tests.
Biological background
This product is centered on measurement of quantiquik pyruvate quick test strips within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
PYRUVATE, a key intermediate in cellular metabolic pathways, can be converted to a variety of things such as carbohydrates, fatty acids, and energy depending on the pathway. It can also be converted to alanine and ethanol. In the beverage industry, monitoring levels of pyruvate during alcoholic fermentation can be important for the production of beer, wine, and spirits. Some brewers even add sodium pyruvate to their beer to alter the flavor and give the beer more body. In regard to health, abnormal levels of pyruvate have been linked to various liver diseases and metabolic disorders. BioAssay Systems QuantiQuik™ Pyruvate Test Strips are based on pyruvate oxidase catalyzed oxidation of pyruvate in which peroxidase reduces the formed peroxide and oxidizes a chromogenic reagent. The intensity of the blue product is directly proportional to pyruvate concentration in the sample.Cat#: QQPYR10
Category: Blood & Urine Chemistry, Food & Beverage Analysis, Quick
Detection method
Visual.
Procedures and timing
Stated procedure or timing information: Approximately 15 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Screen quantiquik pyruvate quick test strips in wine, beer, biological samples using strip-based detection.
- Compare threshold or presence-call outcomes across matched sample groups.
- Monitor routine or field-collected batches with consistent collection timing.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What samples have you tested?
White wine, beer, serum, plasma, and urine.
How sensitive are the Pyruvate strips?
The Strips can measure levels as low as 50 µM of undiluted pyruvate; however, most samples will require some sort of dilution. The following are the detection limits for samples requiring a dilution:• Samples requiring a 2× dilution (e.g. serum and plasma): 100 µM, 8.7 mg/L pyruvate• Samples requiring a 5× dilution (e.g. beer, acidic samples, etc): 250 µM, 22 mg/L pyruvate• Samples requiring a 9× dilution (e.g. white wine and urine): 450 µM, 39 mg/L pyruvate
My sample turns the strip very dark blue, how can I determine the pyruvate concentration?
Very dark blue indicates that the diluted sample concentration is greater than 1.5 mM (131 mg/L or ppm) pyruvate. To obtain a more accurate concentration, the sample should be diluted and retested. For example, if the sample was diluted 2×, try diluting 5× or 9×. Multiply the concentration of the corresponding color by the dilution factor to determine the pyruvate concentration.
I don’t have access to a pipetteman. How can I accurately measure out my samples?
We offer exact volume transfer pipettes as an accessory. For samples requiring a 2× dilution, order the 400 µL transfer pipettes (TP400). For samples requiring a 5× dilution, order the 100 µL transfer pipettes (TP100). For samples requiring a 9× dilution, order the 50 µL transfer pipettes (TP50).
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, preferably cool location protected from light.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
