RACK1 Antibody, HRP conjugated

Overview

This is a polyclonal anti-RACK1 antibody raised in Rabbit, conjugated with HRP, with confirmed utility in ELISA. It is designed to detect RACK1 protein in Human and supports researchers working in cell biology contexts.

Key elements and design rationale

• Immunogen: Recombinant Human Receptor of activated protein C kinase 1 protein (93-317AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
• Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
• Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
• Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
• Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
• HRP conjugate: Direct detection with TMB/OPD or ECL substrates. Quench endogenous peroxidase before use in tissue assays.

Biological background

RACK1 (also referred to as Cell proliferation-inducing gene 21 protein, GBLP_HUMAN, Gnb2-rs1, Gnb2l1, Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1, Guanine nucleotide binding protein beta polypeptide 2 like 1, Guanine nucleotide binding protein beta subunit 2 like 1, Guanine nucleotide binding protein beta subunit like protein 12.3, Guanine nucleotide binding protein subunit beta 2 like 1, Guanine nucleotide binding protein subunit beta like protein 12.3, Guanine nucleotide-binding protein subunit beta-2-like 1, Guanine nucleotide-binding protein subunit beta-like protein 12.3, H12.3, HLC-7, Human lung cancer oncogene 7 protein, lung cancer oncogene 7, OTTHUMP00000223704, OTTHUMP00000223870, OTTHUMP00000223891, OTTHUMP00000223893, OTTHUMP00000223900, OTTHUMP00000223902, OTTHUMP00000223930, OTTHUMP00000223931, PIG21, Proliferation inducing gene 21, Protein homologous to chicken B complex protein guanine nucleotide binding, RACK1, Receptor for activated C kinase 1, Receptor for activated C kinase, Receptor of activated protein kinase C 1) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of RACK1 biology in cell biology.

Common research applications

• ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.

Notes for experimental interpretation

• Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
• Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
• Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
• Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.

This product is a polyclonal antibody purified from rabbit antiserum.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.