RAJI cell

SKU:BHC11100967
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Overview
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RAJI cell is a Lymphoblast cell line derived from African, Nigerian (Male). It is commonly used as an in vitro model for 1 research. Growth characteristics: Suspension. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Disease model Burkitt lymphoma
Growth Properties Suspension
Tissue Maxilia
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Catalog no. Size
300359 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 300359
Species Human
Raji cells are a line of lymphoblast-like cells established by R.J.V. Pulvertaft in 1963 from Burkitt's lymphoma. These cells are widely used in immunology research due to their high expression of human CD19, which acts as a co-receptor and decreases the threshold for antigen B-cell receptor (BCR) stimulation. Raji cells are non-adherent and grow in suspension as free-floating individuals or doublets. The doubling time of these cells is 23.2 hours, and they are relatively small in diameter with a diameter range of 5-8 μm. Some characteristics of Raji cells include a lack of differentiation, as they form large aggregations of hundreds of individual cells. These cells are diploid and have a stable karyotype within the male diploid stemline of 46. Additionally, Raji cells are partially resistant to poliovirus and vesicular stomatitis viruses. Human CD19 is highly expressed by Raji cells and has been identified as a clinical target for anti-hCD19-CD3 bis-specific antibodies in non-Hodgkin's B cell lymphoma. BCMA expression has also been identified in the Raji Burkitt lymphoma cell line and primary lymphoma, making it an important area of research for immunologists.

SKU:BHC11100967

Products: The cells may produce interferon when stimulated by Newcastle disease virus.

  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% heat-inactivated FBS
  • subculturing: Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 105 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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