| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin + Blasticidin |
| Shipping | |
| Species |
Background
RANK (receptor activator of NF-κB, TNFRSF11A) is a TNF receptor superfamily member essential for osteoclast formation and bone remodeling. Engagement by its ligand RANKL, expressed by osteoblasts and stromal cells, drives the differentiation and activation of osteoclasts that resorb bone. RANK signaling activates the NF-κB pathway, a master regulator of differentiation and inflammatory gene expression, so NF-κB response elements provide a quantitative readout of RANK engagement. The RANK-RANKL-osteoprotegerin axis controls skeletal homeostasis and is dysregulated in osteoporosis, bone metastasis, and inflammatory bone loss, making RANK an important target in bone and immune research.
Product Description & Applications
The RANK/NFκB Reporter Lentivirus Set is a two-vial immunotherapy reporter system for studying RANK signaling and osteoclast differentiation. One vial constitutively expresses the human RANK receptor with an antibiotic selection marker, while the other carries tandem NF-κB response elements driving a dual reporter combining secreted Gaussia luciferase (GLuc) and a fluorescent protein (eGFP or mCherry). Sequential transduction and selection generate a dual-stable cell line that responds quantitatively to RANK engagement. Secreted GLuc accumulates in conditioned media for kinetic, lysis-free sampling, while the fluorescent reporter enables microscopy and flow cytometry. Applications include osteoclast differentiation assays and studies of bone remodeling and immune signaling. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human RANK under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (Gaussia Luciferase + eGFP, Gaussia Luciferase + mCherry). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
