| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras-specific guanine nucleotide-releasing factor 1 (Ras-GRF1)|Guanine nucleotide-releasing protein (GNRP)|Ras-specific nucleotide exchange factor CDC25|RASGRF1|CDC25|GNRP|GRF1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Ras protein-specific guanine nucleotide-releasing factor 1 |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes RASGRF1. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:500 - 1:2000 |
| IHC | 1:50 - 1:200 |
| IF | 1:50 - 1:100 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Ras protein-specific guanine nucleotide-releasing factor 1
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Immunogen | Ras protein-specific guanine nucleotide-releasing factor 1 |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 145 kDa |
| UniProt # | Q13972 |
| Gene ID | 5923 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Ras-specific guanine nucleotide-releasing factor 1 (Ras-GRF1)|Guanine nucleotide-releasing protein (GNRP)|Ras-specific nucleotide exchange factor CDC25|RASGRF1|CDC25|GNRP|GRF1 |
Reactivity Notes
Validated reactivity: Human, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
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Explore related antibodies and reagents for RASGRF1 research from Fine Test, or contact us for alternative formats.
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