| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit |
| UniProt # | |
| Species | |
| Reactivity | |
| Applications | |
| Assay type | |
| Sample type(s) | serum, plasma, tissue homogenates, cell culture supernates |
| Sensitivity | |
| Detection range | |
| Detection wavelength | |
| Assay time | |
| Catalog no. (Mfr.) | |
| Main SKU |
Background
5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) is a biological molecule commonly studied in cardiovascular research. It is commonly used as a molecular readout in mechanistic and biomarker-focused studies.
UniProt: P21588
Biological context
Researchers often monitor 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) in serum, plasma, tissue homogenates, and cell culture supernates to better understand themes such as vascular biology and endothelial function, cardiac remodeling and injury responses, and thrombosis and hemostasis. In many model systems, measured levels can shift with physiology, experimental perturbation, or disease-associated changes, making careful biological interpretation important.
Interpreting changes in measured levels
Depending on sample matrix and study design, increases or decreases in 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) may reflect differences in expression, secretion, turnover, or compartmentalization rather than a single mechanism. Interpretation is typically strengthened by evaluating related molecules (for example, endothelial markers, coagulation-related proteins, and cardiac injury markers) and by keeping pre-analytical variables consistent across groups.
Nomenclature
In publications and databases, 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) may also appear under names such as Nt5e and Nt5. When comparing studies, confirm that the reported analyte refers to the same molecule and species context.
Why ELISA data are widely used
ELISA is a common approach for quantitative measurement of proteins and biomarkers in complex samples, enabling comparisons across experimental groups and time points. When integrating results with other readouts, consider species biology, sample type, and the broader pathway context that 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) participates in.
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