| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-1;ANG-1;Angpt1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: H16-F498 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies rat angiopoietin-1 (gene Angpt1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Angiopoietin-1 (ANGPT1) is a secreted ligand that activates the endothelial receptor tyrosine kinase TIE2 (TEK). TIE2 signaling from ANGPT1 promotes endothelial survival and vessel maturation and keeps mature vessels stable and less leaky, so ANGPT1 is studied in angiogenesis, heart development and vascular disease.
Kit Components
| Anti-Rat Angpt1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Rat Angpt1 Standard | 2 vials, 10 ng/tube |
| Rat Angpt1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Rat |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant rat Angpt1 (His16–Phe498), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.7–6.1% (3 samples, n = 16 each) |
| Inter-assay CV | 5.5–6.5% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:100. In the supplier’s tests, serum and plasma samples measured about 120 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.04; 156 = 0.109; 312 = 0.198; 625 = 0.311; 1250 = 0.685; 2500 = 1.131; 5000 = 1.69; 10000 = 2.289. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.04 | 0.109 | 0.198 | 0.311 | 0.685 | 1.131 | 1.69 | 2.289 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 311 | 1285 | 4930 | 287 | 1289 | 5041 |
| Standard deviation | 18.97 | 60.39 | 295.8 | 18.65 | 70.89 | 317.58 |
| CV (%) | 6.1% | 4.7% | 6% | 6.5% | 5.5% | 6.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Heyba Mohammed et al. (2019) Viability and Contractility of Rat Brain Pericytes in Conditions That Mimic Stroke; an in vitro Study. Frontiers in Neuroscience. 10.3389/fnins.2019.01306
Yang Xue et al. (2019) Osteogenic and angiogenic characterization of mandible and femur osteoblasts. Journal of Molecular Histology. 10.1007/s10735-019-09810-6
Zeinab Adel Samy et al. (2018) Rat astrocytes during anoxia: Secretome profile of cytokines and chemokines. Brain and Behavior. 10.1002/brb3.1013
Jian-Jie Rong et al. (2015) Biocompatibility of porcine small intestinal submucosa and rat endothelial progenitor cells in vitro. International Journal of Clinical and Experimental Pathology. PMID: 25973012
