| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bdnf; Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: H129-R247 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This one-step sandwich ELISA quantifies rat neurotrophic factor BDNF precursor form in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). The strips are pre-coated with a capture antibody; sample and an HRP-linked detection antibody are incubated together, so the assay needs fewer wash and incubation steps than a standard sandwich ELISA (the supplier markets the Quick format as a 90-minute assay). TMB develops color in proportion to the bound target, and the plate is read at 450 nm.
Brain-derived neurotrophic factor (BDNF) is a neurotrophin secreted as proBDNF and processed to mature BDNF, which signals mainly through the TrkB receptor (NTRK2). It supports survival and differentiation of neurons during development and regulates synaptic plasticity, learning and memory in the adult brain, making it a common target in neuroscience and psychiatry research.
Kit Components
| Anti-Rat Bdnf Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Rat Bdnf Standard | 2 × 10 ng/tube |
| HRP Linked Anti-Rat Bdnf Antibody | 6 mL |
| Sample Diluent | 15 mL |
| TBS-T Wash Buffer (25x) | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Adhesive Plate Sealers | 2 |
Also required (not supplied): microplate reader for 450 nm; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution; horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3 mm.
Kit components are not sold separately.
Performance Data
| Species | Rat |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <15 pg/mL |
| Standard | Recombinant rat Bdnf (His129–Arg247), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.2–7.7% (3 samples, n = 16 each) |
| Inter-assay CV | 5.4–8.4% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.014; 31.2 = 0.039; 62.5 = 0.076; 125 = 0.120; 250 = 0.255; 500 = 0.485; 1000 = 1.020; 2000 = 1.955. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months. Ships on gel ice and tolerates up to 3 days at room temperature; refrigerate on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.014 | 0.039 | 0.076 | 0.120 | 0.255 | 0.485 | 1.020 | 1.955 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 95 | 302 | 947 | 88 | 289 | 1024 |
| Standard deviation | 4.94 | 16.91 | 72.91 | 4.75 | 21.67 | 86.01 |
| CV (%) | 5.2% | 5.6% | 7.7% | 5.4% | 7.5% | 8.4% |
Kit components
Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|PieceMaterials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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