| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fibroblast growth factor |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Expression System | |
| Gene ID | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: Y30-S208 |
| Product Type | |
| Reactivity | |
| Sample Type(s) | cell culture supernatants, serum and plasma (heparin, EDTA). |
| Sensitivity | |
| Storage | |
| Target | |
| UniProt # |
Background
Also known as: Fibroblast growth factor.
Rat FGF21 (FGF21) is an established target in many assay panels, supporting hypothesis testing across diverse biological systems. This target is frequently investigated in Molecular & Cellular Biology research contexts. Growth factors and morphogens regulate cell proliferation, differentiation, survival, and tissue remodeling by engaging surface receptors and activating downstream signaling cascades. Their activity is often context-dependent, shaped by receptor availability, extracellular matrix binding, and feedback regulation.
Biological function and mechanism
In many systems, growth-factor signaling integrates environmental cues with developmental or repair programs. Downstream pathways frequently include kinase signaling modules and transcriptional responses that alter cell-cycle control, migration, or lineage specification. Because these signals can be transient, quantitative measurements are useful for understanding timing and dose dependence.
Why it matters in research
- Pathway engagement: Concentration changes can indicate activation of growth, survival, or differentiation programs.
- Tissue remodeling: Levels may relate to repair, fibrosis, angiogenesis, or developmental patterning in model systems.
- Mechanistic studies: Tracking abundance alongside downstream markers helps connect ligand availability to signaling output.
Disease and translational relevance
Altered growth-factor signaling has been reported across diverse conditions, including cancer biology, cardiovascular remodeling, wound repair, and metabolic dysfunction. For research interpretation, consider whether the measured form represents active ligand, bound complexes, or processed fragments, as these can influence apparent levels.
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.071 | 0.144 | 0.207 | 0.352 | 0.586 | 0.956 | 1.569 | 2.251 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 50 | 155 | 638 | 57 | 172 | 749 |
| Standard deviation | 2.1 | 6.67 | 30.62 | 2.79 | 11.01 | 58.42 |
| CV (%) | 4.2% | 4.3% | 4.8% | 4.9% | 6.4% | 7.8% |
Kit components
| Catalog number | Description | Quantity | Storage of opened/reconstituted material |
|---|---|---|---|
| EZ1595-CA | Rabbit anti- rat Fgf21 polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
| EZ1595-DA | Biotinylated goat anti- rat Fgf21 polyclonal antibody (Detection Antibody) | 500 µl, 2.1 µg/mL (recommended dilution 1:100) | |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
| EK1595-ST | Lyophilized recombinant rat Fgf21 standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
Materials required but not provided
- Microplate reader in standard size.
- Automated plate washer.
- Incubator.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- 96 well microplate (Cat# AR1100)
- Plate Sealers.
- Capture Antibody Diluent: PBS.
- Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- Stop Solution: 2 N H2SO4 (Cat# AR1105)
- Wash Buffer (PBS and PBS-T). Recipes:
- PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
- PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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