| Field | Specification |
|---|---|
| Analyte | |
| Analyte category | |
| Organism | |
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Sample type(s) | Serum, Plasma, Cell Culture Supernatant |
| Detection method | |
| Detection instrument(s) | Flow Cytometer (Luminex-compatible or equivalent) |
| Instrument required | |
| Plate format | |
| Assay time | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Flow Cytometry Assay components to measure Rat IFN Gamma in Rat Serum, Plasma, Cell Culture Supernatant or Cell Lysate samples. Can be custom Multi-Plexed with other assays in Rat Group 1 ( Choose from 19 assays!)
Panel
Rat IFN Gamma
Note
Order the matching ANTIGENIX Starter Kit (STKM10) And Sample Diluent Kit ( to match sample type): Mouse/ Rat Serum/Plasma (DKM100)or Cell Culture Supernatant (DKC100) or Cell Lysate (DKL100) Cell Lysate Prep Buffer (CLP200) or Sample Dilution Buffer ( to dilute high concentration samples) -(SDL200) also available
Kit Contents
- Antibody Conjugated Beads
- Detection Antibody - Biotin
- Standard: One vial of lyophilized standard containing
- 100 uL ( 45X) (Mouse Group 1)
- 100 uL (25X)
- Pre-mixed multiplexed analytes ( see vial insert) -single
- standard vial can be used for one or more assays indicated
STARTER Kit Included
- (STKM10)
- And Sample Diluent Kit ( to match sample type):
- Mouse/ Rat Serum/Plasma (DKM100)or
- Cell Culture Supernatant (DKC100) or
- Cell Lysate (DKL100)
- Cell Lysate Prep Buffer (CLP200) or Sample Dilution Buffer
- ( to dilute high concentration samples) -(SDL200) also available CONTENTS:
- Antibody Conjugated Beads
- Detection Antibody - Biotin
- Standard: One vial of lyophilized standard containing
- 100 uL ( 45X) (Mouse Group 1)
- 100 uL (25X)
- Pre-mixed multiplexed analytes ( see vial insert) -single
- standard vial can be used for one or more assays indicated
Assay Performance
- Sample Volume: 15.0 uL per test
- Intra-Assay CV: <10%
- Inter-Assay CV: <20%
- Recovery: 70-130%
Storage
Store Refrigerated ( 4-8°C.)
⚠ Caution
Dispose contents carefully, flush with water to avoid reaction with lead drain lines.
| Parameter | Value |
|---|---|
| Sample Volume | 15.0 µL per test |
| Intra-Assay CV | <10% |
| Inter-Assay CV | <20% |
| Recovery | 70–130% |
| Cross-Reactivity (within panel) | Negligible |
| Approximate Assay Time | ~3 Hours |
Performance data sourced from Antigenix America product datasheet. Actual values may vary by lot. Refer to certificate of analysis supplied with kit.
What sample types are compatible?
This Rat Super-X Plex™ assay is compatible with serum, plasma, cell culture supernatant, and cell lysate samples. A species-matched Sample Diluent Kit (DKM100 (Mouse/Rat Serum/Plasma)) must be ordered separately.
What instrument is required?
A flow cytometer capable of detecting PE fluorescence is required. The assay is compatible with Luminex-based platforms and standard flow cytometers with bead-based multiplex detection capability.
What analytes are included in this panel?
IFN Gamma.
How much sample is needed per test?
15 µL per test. 96 Tests kit provides sufficient reagents for a full standard curve plus samples.
Looking for a specific analyte, species, or panel configuration not listed? The Super-X Plex™ platform is designed for flexibility — from off-the-shelf single-plex assays to fully configured custom multi-plex panels and bespoke kit builds.
Custom Multi-Plex Panel Design
Individual Super-X Plex™ assays within the same species group can be combined into custom multiplex configurations without additional cross-reactivity validation. Select any combination of analytes from the available group catalog — from 2-plex to 24-plex — and receive a single pre-mixed standard and matched bead set. Custom panels are available for Human, Mouse, Rat, Canine, and Non-Human Primate matrices.
Custom Kit Services
Beyond panel configuration, BioHippo and Antigenix America offer extended custom kit services to support specialized study designs:
- Analyte selection: Access to the full Super-X Plex™ catalog, including analytes not listed online. If your target is not shown, contact us — additional cytokines, chemokines, growth factors, and soluble receptors may be available.
- Species expansion: Custom assay development for species beyond the standard catalog (Equine, Feline, Porcine, Bovine, and others) may be available upon inquiry.
- Sample matrix compatibility: Assays are validated for serum, plasma, cell culture supernatant, and cell lysate. Custom matrix validation or adjusted diluent formulations can be explored for non-standard sample types.
- Kit size and format: Standard kits are supplied as 96-test formats. Smaller pilot formats (32-test) are available for select panels. Bulk quantities and custom packaging for high-throughput or multi-site studies can be quoted on request.
- Pre-mixed standard panels: Custom pre-mixed lyophilized standards can be formulated to match your exact panel composition, simplifying standard curve preparation across multiple assay runs.
- White-label and OEM supply: For core facilities, CROs, or institutional programs requiring custom-labeled or privately packaged kits, OEM supply arrangements can be discussed.
How to Request
Click Talk to a Scientist to submit a customization request form, email us at support@biohippo.com, or explore our Research Services for additional support options. Our team will review your requirements and respond promptly.
