| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Sensitivity | |
| Target | |
| UniProt # |
Scientific background
IL1b (Interleukin 1 Beta) is an interleukin-family cytokine that helps coordinate immune cell communication and inflammatory responses.
Interleukin levels often shift quickly after immune stimulation, making them useful for time-course studies of inflammation and immune activation.
Quantifying interleukins can help interpret pathway engagement alongside phenotypic readouts (e.g., immune cell infiltration, fever, or tissue injury markers).
Why it matters
- Quantify IL1b (Interleukin 1 Beta) to compare biological changes across conditions, doses, or time points.
- Generate concentration data from a standard curve to support biomarker and mechanistic studies.
How the ELISA works
Designed for Rat samples, this kit uses a The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.. After binding and washing, signal is converted to concentration using a standard curve.
Sample types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Detection range: 15.63-1000 pg/mL
- Sensitivity/LoD: 5.9 pg/mL
- Assay time: 3.5h
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