| Field | Specification |
|---|---|
| Target | |
| Alternative names | Laminin subunit beta-2;Laminin chain B3;Laminin-11 subunit beta;Laminin-14 subunit beta;Laminin-15 subunit beta;Laminin-3 subunit beta;Laminin-4 subunit beta;Laminin-7 subunit beta;Laminin-9 subunit beta;S-laminin subunit beta;S-LAM beta;Lamb2; |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Target protein isolated from rat sarcoma basement membrane |
| Expression system | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies rat basement membrane-specific heparan sulfate proteoglycan core protein (gene Lama1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Laminin alpha-1 (LAMA1) is a subunit of laminin-111, one of the first laminins expressed in the embryo and a major component of early basement membranes. By binding integrins and alpha-dystroglycan, it supports cell adhesion, migration and tissue organization, and it takes part in epithelial branching morphogenesis. LAMA1 is studied in development, basement membrane biology and stem cell culture.
Kit Components
| Anti-Rat Laminin Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Rat Laminin Standard | 2 vials, 10 ng/tube |
| Rat Laminin Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Rat |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.4–7.4% (3 samples, n = 16 each) |
| Inter-assay CV | 5.5–8.5% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:10. In the supplier’s tests, serum samples measured 5.2–11.2 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.021; 156 = 0.103; 312 = 0.176; 625 = 0.332; 1250 = 0.554; 2500 = 0.935; 5000 = 1.423; 10000 = 1.932. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.021 | 0.103 | 0.176 | 0.332 | 0.554 | 0.935 | 1.423 | 1.932 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 256 | 1435 | 4770 | 279 | 1524 | 4389 |
| Standard deviation | 11.26 | 97.58 | 352.98 | 15.34 | 128.01 | 373.06 |
| CV (%) | 4.4% | 6.8% | 7.4% | 5.5% | 8.4% | 8.5% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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