| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;Ntf3;Ntf-3; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Y139-T257 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies rat neurotrophin-3 (gene Ntf3) in cell culture supernatants and serum. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Neurotrophin-3 (NT-3, NTF3) is a secreted growth factor of the neurotrophin family that acts mainly through the TrkC receptor. It supports the survival and development of sensory neurons, including proprioceptive and visceral populations, and is studied in nervous system development, nerve injury and synaptic plasticity.
Kit Components
| Anti-Rat Ntf3 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Rat Ntf3 Standard | 2 vials, 10 ng/tube |
| Rat Ntf3 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Rat |
|---|---|
| Sample types | Cell culture supernatants and serum |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <2 pg/mL |
| Standard | Recombinant rat Ntf3 (Tyr139–Thr257), expressed in NS0 |
| Cross-reactivity | Below 2.5% cross-reactivity with NT-4; none detected with any other cytokine |
| Intra-assay CV | 5.5–7.2% (3 samples, n = 16 each) |
| Inter-assay CV | 5.5–9.0% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.05; 15.6 = 0.132; 31.2 = 0.204; 62.5 = 0.374; 125 = 0.645; 250 = 1.118; 500 = 1.865; 1000 = 2.042. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.05 | 0.132 | 0.204 | 0.374 | 0.645 | 1.118 | 1.865 | 2.042 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 41 | 173 | 464 | 41 | 177 | 504 |
| Standard deviation | 2.25 | 12.45 | 23.66 | 2.99 | 15.53 | 27.72 |
| CV (%) | 5.5% | 7.2% | 5.5% | 7.3% | 9% | 5.5% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Ge Li et al. (2021) An NT-3-releasing bioscaffold supports the formation of TrkC-modified neural stem cell-derived neural network tissue with efficacy in repairing spinal cord injury. Bioactive Materials. 10.1016/j.bioactmat.2021.03.036
Qi Sha et al. (2023) A hyaluronic acid/silk fibroin/poly-dopamine-coated biomimetic hydrogel scaffold with incorporated neurotrophin-3 for spinal cord injury repair. Acta Biomaterialia. 10.1016/j.actbio.2023.05.044
Ge Li et al. (2015) Graft of the NT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration, attenuates inflammation, and induces cell migration in rat and canine with spinal cord injury. Biomaterials. 10.1016/j.biomaterials.2015.11.059
Qiang Chen et al. (2021) Triptolide improves neurobehavioral functions, inflammation, and oxidative stress in rats under deep hypothermic circulatory arrest. Aging-US. 10.18632/aging.202460
Ding Ying et al. (2015) Combination of Electroacupuncture and Grafted Mesenchymal Stem Cells Overexpressing TrkC Improves Remyelination and Function in Demyelinated Spinal Cord of Rats. Scientific Reports. 10.1038/srep09133
Haoyu Xu et al. (2021) Governor Vessel Electro-Acupuncture Promotes the Intrinsic Growth Ability of Spinal Neurons through Activating Calcitonin Gene-Related Peptide/α-Calcium/Calmodulin-Dependent Protein Kinase/Neurotrophin-3 Pathway after Spinal Cord Injury. Journal of Neurotrauma. 10.1089/neu.2020.7155
JINGE TIAN et al. (2017) Swimming Training Reduces Neuroma Pain by Regulating Neurotrophins. Medicine & Science in Sports & Exercise. 10.1249/MSS.0000000000001411
Wenming Yin et al. (2024) Mussel shell-derived pro-regenerative scaffold with conductive porous multi-scale-patterned microenvironment for spinal cord injury repair. Biomedical Materials. 10.1088/1748-605X/ad3f63
Zhe Zhao et al. (2014) Improvement in Nerve Regeneration through a Decellularized Nerve Graft by Supplementation with Bone Marrow Stromal Cells in Fibrin. Cell Transplantation. 10.3727/096368912X658845
Xiaoyu Wang et al. (2025) Enhanced Bone Regeneration by Schwann Cells through Coupling of Osteogenesis and Angiogenesis via β-catenin signaling in a Preclinical Model of Distraction Osteogenesis. International Journal of Medical Sciences. 10.7150/ijms.100854
