| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Disease | Normal tissue |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Rat Primary Esophageal Smooth Muscle Cells are rat frozen primary cells from mouth/oral used for vascular remodeling, contractile signaling, and smooth muscle phenotypic regulation studies. These cells display adherent, multipolar growth characteristics.
Key elements and design rationale
- Biological source: Rat (R. norvegicus)
- Tissue origin: Mouth/Oral
- Growth properties: Adherent, multipolar
- Format: Frozen
- Reported expression/markers: Alpha-actin
- Seeding guidance: 6,000 - 10,000 cells/cm²
- Biosafety level: II
Smooth muscle cells regulate vessel tone, extracellular matrix remodeling, and responses to growth factors, cytokines, and mechanical cues. In culture, they are often used to study contractile-to-synthetic state transitions.
Research relevance and current trends
- Studies frequently examine how inflammatory, mechanical, or metabolic cues alter smooth muscle phenotype and matrix production.
- Primary vascular smooth muscle cells are used to model remodeling pathways relevant to stenosis, calcification, and vascular injury.
- Comparative donor and vessel-source studies help capture regional heterogeneity across the arterial tree.
Common research applications
- Measure contractile marker expression or phenotype switching after defined perturbations.
- Evaluate proliferation, migration, and matrix-remodeling responses in vascular remodeling models.
- Use in co-culture with endothelial cells or fibroblasts to examine vessel wall signaling.
Product-specific data supplied for this listing
- Growth Conditions: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4913) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Seeding Density (cells/cm²): 6,000 - 10,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Marker panels should be interpreted together with morphology and functional readouts rather than as a standalone identity measure.
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
