| Field | Specification |
|---|---|
| Target | |
| Alternative names | FLJ10764; MEGF5; SLIL2; SLIT1; Slit-3; slit2; slit homolog 3 |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The SLIT3 cDNA encodes a 1,523-amino acid polypeptide with 41.1% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT3 gene was expressed as a major 5.5- and a minor 9.5-kb transcript primarily in the thyroid. SLIT1 and SLIT2 mRNAs were primarily expressed in the brain and spinal cord, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate SLIT3 in samples. An antibody specific for SLIT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.063 ng/mL |
| Species Reactivity | Rat (Rattus norvegicus) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE18232RA |
| UniProt # | O88280 |
| Alternative Names | FLJ10764; MEGF5; SLIL2; SLIT1; Slit-3; slit2; slit homolog 3 |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Rat ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for SLIT3 research.
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