Rat TGF Beta 1 ELISA Kit PicoKine®

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Rat TGF Beta 1 (TGFB1) PicoKine® Quick ELISA kit is designed for quantitative detection of TGF Beta 1. Suitable for cell culture supernatants, serum, plasma (EDTA) and urine. Optimized for a streamlined sandwich ELISA workflow with high signal-to-noise and reproducible results. Reported sensitivity: <1 pg/ml.
Target TGFB1
Reactivity Rat
Sample Type(s) cell culture supernatants, serum, plasma (EDTA) and urine.
Assay Type Sandwich ELISA
Sensitivity <1 pg/ml
Detection Range 15.6 pg/ml - 1,000 pg/ml
Assay Time ~3.5 hours
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Options selector
Catalog no. Size
EK0514 96 wells/kit, with removable strips.
Available Options

Select from the available variant options shown for this product. Availability and lead time may vary by option.

  • Options: Size: 96 wells/kit, with removable strips..
  • Lead time: items “in stock at manufacturer” typically ship in 5–7 business days.
  • Storage: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.); cold-chain shipment (typically with ice packs) is expected.
  • Please ensure someone is available to receive temperature-sensitive deliveries promptly.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No EK0514
Alternative Names Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;Tgfb1;
Assay Time
  • ~3.5 hours
Assay Type
  • Sandwich ELISA
Detection Range 15.6 pg/ml - 1,000 pg/ml
Expression System
  • CHO
Gene ID 59086
Immunogen Expression system for standard: CHO; Immunogen sequence: A279-S390
Product Type
  • ELISA Kits
  • PicoKine® ELISA Kitss
Reactivity
  • Rat
Sample Type(s) cell culture supernatants, serum, plasma (EDTA) and urine.
Sensitivity <1 pg/ml
Storage Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Target TGFB1
UniProt # P17246

Background

Also known as: Transforming growth factor beta-1, TGF-beta-1, Latency-associated peptide, LAP, Tgfb1.

Rat TGF Beta 1 (TGFB1) is a commonly measured biological analyte that can provide insight into cellular state and tissue physiology. This target is frequently investigated in Immunology & Inflammation research contexts. Growth factors and morphogens regulate cell proliferation, differentiation, survival, and tissue remodeling by engaging surface receptors and activating downstream signaling cascades. Their activity is often context-dependent, shaped by receptor availability, extracellular matrix binding, and feedback regulation.

Biological function and mechanism

In many systems, growth-factor signaling integrates environmental cues with developmental or repair programs. Downstream pathways frequently include kinase signaling modules and transcriptional responses that alter cell-cycle control, migration, or lineage specification. Because these signals can be transient, quantitative measurements are useful for understanding timing and dose dependence.

Why it matters in research

  • Pathway engagement: Concentration changes can indicate activation of growth, survival, or differentiation programs.
  • Tissue remodeling: Levels may relate to repair, fibrosis, angiogenesis, or developmental patterning in model systems.
  • Mechanistic studies: Tracking abundance alongside downstream markers helps connect ligand availability to signaling output.

Disease and translational relevance

Altered growth-factor signaling has been reported across diverse conditions, including cancer biology, cardiovascular remodeling, wound repair, and metabolic dysfunction. For research interpretation, consider whether the measured form represents active ligand, bound complexes, or processed fragments, as these can influence apparent levels.

Sample data

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0480.1190.1770.2990.4920.8571.4632.223

Intra/inter assay consistency

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2716143128156422
Standard deviation1.599.8223.71.739.9826.16
CV (%)5.5%6.1%5.5%6.2%6.4%6.2%

Kit components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Materials required but not provided

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Activating reagent preparation

TGF beta 1 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGF beta 1.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H2O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml.

Sample activation procedure

  • Activate the sample

    Cell culture supernatants, urine: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6.

    Serum, plasma(EDTA): add activating reagent pro rata, i.e. add 20μl of Solution A into 40μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6.

    It is unnecessary to activate the recombinant TGFβ 1.

    Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

    • ►How many samples can I run per plate?
    Each PicoKine® kit (96-well format) typically accommodates a 7-point standard curve in duplicate, 2 non-specific binding wells, and up to 39 unknown samples in duplicate. Exact capacity may vary by kit — refer to the datasheet.
    ►What sample dilution should I use?
    Boster recommends performing a pilot study first: run serial dilutions of your samples (e.g. 1:2, 1:4, 1:8, 1:16) to identify the range that falls within the standard curve. This is important because sample matrix and protein expression levels vary significantly by experiment.
    ►Why is my signal weak or absent?
    The most common causes are: (1) target protein below the kit's detection limit — try concentrating samples or reducing dilution; (2) reagents not at room temperature before use; (3) insufficient incubation time; (4) expired or contaminated reagents. See Boster's full ELISA troubleshooting guide at bosterbio.com/protocol-and-troubleshooting/picokine-elisa-troubleshooting.
    ►Why is my background signal high?
    High background is typically caused by insufficient washing (ensure thorough plate draining after each wash step), excess antibody concentration, or contaminated TMB substrate. Increase the number of wash cycles or reduce antibody concentration. Always use fresh substrate solution.
    ►Are the kit components sterile?
    Components are bottled using aseptic techniques and heat-treated vials, but are not guaranteed sterile. If your experiment requires sterile material, filter through a 0.2 µm membrane designed for biological fluids before use.
    ►How do I analyze my ELISA results?
    Plot absorbance (OD 450 nm) against standard concentrations and fit a 4-parameter logistic (4PL) or sigmoidal curve. Read unknown sample concentrations from the curve. Boster provides a free online ELISA data analysis tool at bosterbio.com/biology-research-tools/elisa-data-analysis-online.
    ►How should I store samples before running the assay?
    Aliquot samples before freezing to avoid repeated freeze-thaw cycles, which can degrade the target protein. Store aliquots at -80°C for long-term use. Serum and plasma should be collected, processed, and stored under consistent conditions to minimise pre-analytical variability.
    ►What positive and negative controls should I include?
    Include a positive control (a sample known to contain the target protein at a measurable level) and a negative control (sample matrix without the target, or a sample from a species the kit does not cross-react with). Running controls in every assay validates the assay performance and flags plate-to-plate variability.

    Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

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