| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor ligand superfamily member 11;Osteoclast differentiation factor;ODF;Osteoprotegerin ligand;OPGL;Receptor activator of nuclear factor kappa-B ligand;RANKL;TNF-related activation-induced cytokine;TRANCE;CD254;Tumor necrosis factor ligand superfamily member 11, membrane form;Tumor necrosis factor ligand superfamily member 11, soluble form;Tnfsf11;Opgl, Rankl, Trance; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: F141-D318 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies rat tumor necrosis factor ligand superfamily member 11 (gene Tnfsf11) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
RANKL (TNFSF11, TRANCE) is a TNF family cytokine that binds its receptor RANK on osteoclast precursors and drives osteoclast formation and bone resorption, largely through calcium oscillations and NFATc1 activation. Its action is opposed by the decoy receptor osteoprotegerin. RANKL also influences dendritic cell and T-cell interactions, and it is central to bone remodeling and osteoporosis research.
Kit Components
| Anti-Rat Tnfsf11 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Rat Tnfsf11 Standard | 2 vials, 10 ng/tube |
| Rat Tnfsf11 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Rat |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant rat Tnfsf11 (Phe141–Asp318), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.1–6.2% (3 samples, n = 16 each) |
| Inter-assay CV | 5.8–7.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 20–200 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.035; 15.6 = 0.058; 31.2 = 0.081; 62.5 = 0.137; 125 = 0.298; 250 = 0.622; 500 = 1.356; 1000 = 1.931. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.035 | 0.058 | 0.081 | 0.137 | 0.298 | 0.622 | 1.356 | 1.931 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 37 | 209 | 464 | 38 | 208 | 445 |
| Standard deviation | 2.25 | 8.56 | 28.76 | 2.96 | 12.06 | 37.95 |
| CV (%) | 6.1% | 4.1% | 6.2% | 7.8% | 5.8% | 7.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Man Zhang et al. (2021) Novel optimized drug delivery systems for enhancing spinal cord injury repair in rats. Drug Delivery. 10.1080/10717544.2021.2009937
Haoran Ning et al. (2019) Microfiber-Reinforced Composite Hydrogels Loaded with Rat Adipose-Derived Stem Cells and BMP-2 for the Treatment of Medication-Related Osteonecrosis of the Jaw in a Rat Model. ACS Biomaterials Science & Engineering. 10.1021/acsbiomaterials.8b01468
Li Xue-dong et al. (2011) Panax notoginseng Saponins Potentiate Osteogenesis of Bone Marrow Stromal Cells by Modulating Gap Junction Intercellular Communication Activities. Cellular Physiology and Biochemistry. 10.1159/000323986
Bin Chen et al. (2012) Canonical Wnt signaling is required for Panax notoginseng saponin-mediated attenuation of the RANKL/OPG ratio in bone marrow stromal cells during osteogenic differentiation. Phytomedicine. 10.1016/j.phymed.2012.06.002
Zhang Benchao et al. (2020) Interference with miR-210 Alleviated Renal Injury in Septic Rats by Inhibiting JAK-STAT Pathway. Inflammation. 10.1007/s10753-020-01283-0
Xuan Su et al. (2018) ‘Governor vessel-unblocking and mind-regulating’ acupuncture therapy ameliorates cognitive dysfunction in a rat model of middle cerebral artery occlusion. International Journal of Molecular Medicine. 10.3892/ijmm.2018.3981
Hong‑Xu Jin et al. (2018) Inhibition of MEK/ERK/STAT3 signaling in oleuropein treatment inhibits myocardial ischemia/reperfusion. International Journal of Molecular Medicine. 10.3892/ijmm.2018.3673
Limeng Shou et al. (2019) Nrf2 mediates the protective effect of edaravone after chlorpyrifos-induced nervous system toxicity. Environmental Toxicology. 10.1002/tox.22728
Xudong Wang et al. (2018) Allicin attenuates lipopolysaccharide‑induced acute lung injury in neonatal rats via the PI3K/Akt pathway. Molecular Medicine Reports. 10.3892/mmr.2018.8693
Limin Liu et al. (2019) Imatinib inhibits oxidative stress response in spinal cord injury rats by activating Nrf2/HO‑1 signaling pathway. Experimental and Therapeutic Medicine. 10.3892/etm.2019.8270
