| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RBP3;D10S64;D10S65;D10S66;IRBP;RBPI;RP66 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant fusion protein of human RBP3(NP_002891.1). |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
RBP3 polyclonal antibody is a polyclonal primary antibody raised in Rabbit against RBP3. It is supplied in unconjugated form. The antibody is documented by Bioworld Technology Inc for use in Western Blot, Immunohistochemistry.
Scientific Background
Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.
Validated Applications & Specifications
| Validated Applications | Western Blot, Immunohistochemistry |
|---|---|
| Recommended Dilution | WB,1:500 - 1:2000|IHC,1:50 - 1:100 |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Predicted Molecular Weight | ~ 180 kDa |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation / Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| UniProt / Accession | P10745 |
Immunogen / Antigen
Recombinant fusion protein of human RBP3(NP_002891.1).
Reactivity Notes
Polyclonal Antibodies
Documented species reactivity: Human, Mouse, Rat.
Quality control: > 95% (by SDS-PAGE)
Safety & Handling
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Related Products
Browse additional primary antibodies and immunodetection reagents from Bioworld Technology Inc available through Biohippo.
This antibody is documented for use in Western Blot, Immunohistochemistry. Recommended dilutions: WB,1:500 - 1:2000|IHC,1:50 - 1:100.
Documented species reactivity is Human, Mouse, Rat. Species not listed have not been validated and should be confirmed with appropriate controls.
The immunogen was: Recombinant fusion protein of human RBP3(NP_002891.1).
This antibody is supplied unconjugated and requires a labelled secondary antibody for detection.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
