Recombinant Chicken Leptin Binding Domain

SKU:BHP11002405
Suppliers
ProSpec-Tany TechnoGene Ltd
ProSpec-Tany TechnoGene Ltd
Details Products
Overview
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RUO recombinant Leptin Binding Domain (Chicken) protein for mechanistic studies and assay development. Supplied as a traceable protein input (E. coli; >99% (SDS-PAGE) purity; sterile-filtered solution; MW 24.5 kDa) for reproducible assay inputs.
Target OBS
Species Chicken
Options selector
Catalog no. Size
cyt-509-20UG 20 ug
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 20 ug
  • Lead time: options listed as “in stock at manufacturer” typically ship in 5–7 business days; other statuses may take longer.
  • Storage: Sterile solutions at 0.5mg/ml or less are stable at 4°C for several months.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: refrigerate upon receipt.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No cyt-509
Alternative Names OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor.
Concentration 0.5 mg/ml
Expression System
  • E. coli
Form Sterile Filtered colorless solution at a concentration of 0.4 mg/ml.
Formulation The protein was filter sterilized and stored at 4°C (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl.
Product Type
  • Proteins & Peptides
  • Cytokines and Growth Factors
  • Hormone & Adipokine
Protein Length 208
Protein Size 24.5 kDa
Purity Greater than 99.0% as determined by:<br>(a) Analysis by SEC-HPLC.<br>(b) Analysis by SDS-PAGE.
Source Escherichia Coli.
Species Chicken
Storage Sterile solutions at 0.5mg/ml or less are stable at 4°C for several months.
Target OBS

Recombinant Chicken Leptin Binding Domain is supplied as a recombinant protein for in vitro research use.

Background

Leptin Receptor is a part of the gp130 family of cytokine receptors that stimulate gene transcription by activating cytosolic STAT proteins. Leptin Receptor plays a role in the regulation of fat metabolism and in novel hematopoietic pathway that is obligatory for normal lymphopoiesis. Leptin Receptorparticipates in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus.Leptin Receptoraffectsspecifically on T lymphocyte responses.

Product format

Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.

Quality notes

Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.

What is the purity of Recombinant Chicken Leptin Binding Domain (Chicken)?
Greater than 99.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE. BioHippo includes a Certificate of Analysis (CoA) confirming purity per lot with every order.
What buffer / formulation is this protein supplied in?
Supplied as: The protein was filter sterilized and stored at 4°C (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl. Reconstitute lyophilized material in sterile ultrapure water or the recommended buffer per the datasheet prior to use.
How should Recombinant Chicken Leptin Binding Domain (Chicken) be stored?
Sterile solutions at 0.5mg/ml or less are stable at 4°C for several months. Prepare single-use aliquots after reconstitution to avoid repeated freeze–thaw cycles.
What expression system was used to produce this protein?
This recombinant protein was expressed in E. coli. The system was selected to achieve high yield, correct folding, and appropriate post-translational modifications.
Is this protein approved for clinical or in vitro diagnostic use?
No. Supplied for Research Use Only (RUO) — not intended for therapeutic applications or in vitro diagnostic procedures.
Can I request a custom size, tag variant, or formulation?
Yes. BioHippo can accommodate custom requests including alternative sizes, His/GST/Fc tag variants, bulk quantities, and custom formulations. See the Customization & Add-ons tab or email support@biohippo.com.

Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

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