| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Carboxypeptidase B, also known as peptidyl-L-lysine (L-lysine) hydrolase or pancreatic carboxypeptidase B, is a specialized enzyme that hydrolyzes the amino group at the C-terminus of basic amino acids in proteins (Lysine (Lys, K), Arginine (Arg, R), and Histidine (His, H)). It has a molecular weight of 33.8 kD and an isoelectric point of 6.0, with an optimal pH range of 7.5 to 9.0. The activity of carboxypeptidase B is subject to competitive inhibition by arginine and lysine, and is inhibited by metal ion chelators such as EDTA.
Yeasen UCF.ME™ Recombinant CPB is expressed in E.coli, produced under GMP regulations, free of any animal-derived components, and without the risk of viral contamination from animal sources. The amino acid sequence is identical to that of rat pancreatic carboxypeptidase B, possessing the same enzymatic properties as the animal-derived enzyme, and can be used as a substitute in various biotechnological processes.
Features
- Strong specificity—A specialized enzyme that hydrolyzes the amino group at the C-terminus of basic amino acids in proteins (Lysine (Lys, K), Arginine (Arg, R), and Histidine (His, H))
- High purity—Purity≥95%.
- Animal free—Recombinantly produced, free from exogenous viral contamination, and no animal-derived materials are used in the production process.
Applications
- Production of recombinant insulin and its analogs.
- Determination of the C-terminal amino acids of proteins.
- Removal of the C-terminal histidine tag from proteins.
- Production of other recombinant polypeptide substances.
- Enzymatic synthesis of certain special compounds.
Specifications
|
Source |
E.coli recombinant expression |
|
Molecular Weight |
Theoretical value 33.8±3.4 kDa |
|
Appearance |
White, off-white, or pale yellow powder |
|
Enzyme Concentration |
≥170 USP units/mg pro |
|
Unit Definition |
At 25℃, pH 7.6, the amount of enzyme that catalyzes 1 μmoL of hippuryl-L-arginine hydrolysis in 1 minute is defined as one unit of enzyme activity. |
|
Quality Assurance |
SDS-PAGE gel detection shows a clear single band of the target protein; no other proteases present, no non-specific cutting. |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
20417ES03 |
20417ES10 |
|
20417 |
UCF.ME™ Recombinant CPB（Powder） |
1 mg |
10 mg |
Storage
Lyophilized powder can be stored at 2 ~ 8℃ for two years.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Production of recombit insulin and its analogs. Determination of the C-terminal amino acids of proteins . Removal of the C-terminal histidine tag from proteins . Production of other recombit polypeptide substances . Enzymatic synthesis of certain special compounds .. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U/mg) is defined as the amount of protease activity per milligram of enzyme protein under standardized assay conditions (substrate concentration, pH, temperature, and incubation time).
This enzyme is produced as Recombinant (E. coli) and is available in GMP grade for regulated applications. This enzyme is manufactured in an ISO 13485-certified UCF.ME™ facility and meets GMP-grade quality standards. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Most serine proteases (e.g., Proteinase K) are inhibited by PMSF (1 mM) and heat-inactivated at 95°C for 10 min. Proteinase K retains activity across a broad pH range (4–12) and tolerates SDS, urea, and other denaturants, making it particularly effective for nucleic acid extraction from challenging samples including FFPE tissue.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
