| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Recombinant Enterokinase (rEK) is a highly pure fragment of the recombinant bovine enterokinase light chain. Its amino acid sequence is identical to that of the bovine enterokinase light chain, featuring enzymatic cleavage sites that are as specific as those found in naturally extracted enterokinase, with cutting sites at Asp-Asp-Asp-Asp-Lys. This enables the removal of the fusion protein located at the N-terminus of the protein, eliminating unnecessary fusion tags. Additionally, rEK exhibits higher cleavage activity compared to the natural enzyme.
The Recombinant Enterokinase is a high-purity, highly active, and highly specific bovine enterokinase produced through recombinant expression in Escherichia coli. Devoid of other proteases, it can effectively cleave fusion proteins over a broad pH range (4.5-9.5) and a wide temperature range. It retains partial activity even in the presence of various detergents and denaturing agents. This product is label-free, and due to its extremely high enzymatic cleavage activity, minimal amounts are needed for cleavage reactions, ensuring no interference with downstream protein applications, making removal unnecessary.
Feature
Specificity: The enzyme exhibits specificity for a particular amino acid sequence, cleaving at the carboxyl-terminal site containing four aspartic acids followed by lysine (DDDDK).
Animal free: Produced through recombinant methods, ensuring no contamination from exogenous viruses. The production process does not involve any animal-derived materials.
Quality stability: Batch production guarantees consistently stable batches, with no variations in quality between different batches.
High purity: Devoid of other proteases, ensuring non-specific cleavage.
Compliance with regulations: Production facilities and environments adhere to relevant regulatory requirements. The manufacturing process adheres to GMP guidelines.
Comprehensive quality documentation: Can provide relevant regulatory support documents as per customer requirements.
Specification
|
Source |
Expressed in E.coli. |
|
Molecular Weight |
25.85 kDa |
|
Appearance |
Clear, colorless liquid. |
|
Concentration |
≥5 U/uL |
|
Activity Definition |
One unit of activity is defined as the amount of enzyme required to cleave 95% of 500 μg of a 30KD fusion protein containing enterokinase cleavage sites, under the following conditions: 25°C, 12-16 hours, in a buffer system consisting of 25 mM Tris-HCl, pH 8.0. |
|
Endotoxin |
＜1 EU/μg |
|
HCP |
≤0.01% |
| Tag | No |
|
Storage Buffer |
50 mM Tris-HCl，pH8.0，250 mM NaCl，2 mM CaCl2，50% Glycerol |
The amount of recombinant protein cleaved per unit of Enterokinase (EK)
|
Suppliers |
Yeasen 5 U/μL |
Thermo 1 U/μL |
NEB 16 U/μL |
|
Activity |
500 μg/U |
20 μg/U |
25 μg/U |
Storage
Store at -25 to -15°C for 1 years.
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Protein Research, Nucleic Acid Purification applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U/mg) is defined as the amount of protease activity per milligram of enzyme protein under standardized assay conditions (substrate concentration, pH, temperature, and incubation time).
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Most serine proteases (e.g., Proteinase K) are inhibited by PMSF (1 mM) and heat-inactivated at 95°C for 10 min. Proteinase K retains activity across a broad pH range (4–12) and tolerates SDS, urea, and other denaturants, making it particularly effective for nucleic acid extraction from challenging samples including FFPE tissue.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
