| Field | Specification |
|---|---|
| Mfr No | |
| Biological Activity | |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Source | Escherichia coli |
| Storage |
Scientific Background
Fibroblast Growth Factor-8 (FGF-8) is a heparin-binding growth factor of the FGF family. There are 4 known forms of FGF8 produced by alternative splicing: FGF8a, FGF-8b, FGF-8e and FGF-8f. The human and mouse FGF8b are identical of aa sequences. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF-8 is required for normal brain, eye, ear and limb development during embryogenesis.
Product Description
E. coli ExpressionRecombinant FGF-8 Human protein is produced using a validated E. coli expression system and supplied as lyophilized powder for long-term stability. Suitable for use in functional bioassays, ELISA standard curves, receptor binding studies, antibody validation, and related research applications.
Protein Specifications
| Expression System | E. coli |
|---|---|
| Molecular Weight | 22.5kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% by SDS-PAGE and HPLC analyses. |
| Endotoxin | < 0.2 EU/μg, determined by LAL method. |
| Physical Form | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O at 100 μg/ml. |
| Storage | Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at … |
Functional Activity
ED50 < 5.0 ng/ml, measured by a cell proliferation assay using 3T3 cells in the presence of 1μg/ml of heparin, corresponding to a specific activity of > 2.0× 10ˆ5 units/mg.
Safety & Handling
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
This protein was produced in E. coli expression system. Expression system selection determines glycosylation profile, folding, and post-translational modifications. For cell-based stimulation assays, verify the expression system matches the glycosylation requirements of your target receptor or pathway.
Reconstituted in ddH2O at 100 μg/ml. Reconstitute in sterile distilled water or PBS at 100 µg/mL as a standard starting concentration. Allow to dissolve at 4°C for 30 minutes without vortexing. Prepare working aliquots in 0.1% BSA carrier protein and store at −80°C.
This protein has been validated for functional bioactivity: ED50 < 5.0 ng/ml, measured by a cell proliferation assay using 3T3 cells in the presence of 1μg/ml of heparin, corresponding to a specific activity of > 2.0× 10ˆ5 units/mg.. Optimal working concentrations may vary depending on your cell type, assay format, and culture conditions. Titrate the protein in a dose-response experiment to determine the optimal concentration for your system.
Endotoxin level is < 0.2 EU/μg, determined by LAL method. as determined by the LAL method. Low endotoxin is critical for cell-based studies because endotoxin activates NF-κB and TLR4 signalling in immune cells, producing artefactual cytokine induction that can completely mask the true biological activity of the recombinant protein.
Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-8 should be stable up to 2 weeks at 4°C or up Upon receipt, immediately store at −80°C. Prepare single-use working aliquots to avoid repeated freeze-thaw cycles. Lyophilized protein is stable for 6–12 months at −80°C from the date of receipt.
BioHippo offers flexible sourcing options for qualified research institutions and partners. The following may be available subject to supplier capabilities and order volume.
- Custom quantities: Bulk pricing or non-standard sizes available for high-throughput screening or scale-up projects.
- Custom formulation: Alternative reconstitution buffers or carrier proteins may be accommodated on request.
- Extended QC data: Additional bioactivity assay data, endotoxin reports, or SEC-HPLC purity profiles available on request.
Contact BioHippo customer support to discuss your requirements.
