| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | ALCAM;Activated leukocyte cell adhesion molecule;CD166;CD166 antigen;MEMD |
| Endotoxin Level | |
| Formulation | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Activated leukocyte cell adhesion molecule (ALCAM), also named as CD166 and MEMD, is a typeI transmembrane glycoprotein of immunoglobulin superfamily, which mediates homotypic and heterotypic interactions between cells. ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocytederived dendritic cells. ALCAM mediates low-affinity adhesion with itself or the cysteine-rich scavenger receptor CD6 to regulate T cell development, immunological synapses(IS), and cell migration through endothelial junctions. ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells.
Alternative names: ALCAM;Activated leukocyte cell adhesion molecule;CD166;CD166 antigen;MEMD
Product Description
Mammalian (HEK293) ExpressionThis recombinant protein is produced using a validated Mammalian (HEK293) expression system and supplied as lyophilized powder with C-Fc affinity tag. Reconstitute as directed on the Certificate of Analysis before use. Suitable for biochemical binding assays, ELISA standard curves, antibody validation, protein interaction studies, and related research applications.
Protein Specifications
| UniProt ID | Q13740 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Expression Region | Trp28-Ala526 |
| Affinity Tag | C-Fc |
| Calculated MW | 82.7 kDa |
| Observed MW | 110-125 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8%… |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -2… |
| Shelf Life | 12 months |
Safety & Handling
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
This protein was produced in Mammalian (HEK293) expression system. The expression system determines the protein post-translational modification profile, folding characteristics, and suitability for specific applications. Mammalian (HEK293) expression is used where native-like glycosylation is required; insect cell and E. coli systems are used for high-yield non-glycosylated production.
This product is supplied as lyophilized powder. Centrifuge the vial briefly before opening, then add the recommended volume of sterile water or the specified buffer and allow to dissolve at 4°C for 30 minutes without vortexing. Prepare single-use working aliquots in 0.1% BSA to minimise freeze-thaw losses of the bulk stock.
Endotoxin is < 1.0 EU per μg of the protein as determined by the LAL method., determined by the LAL chromogenic method. The <1.0 EU/µg specification ensures suitability for cell culture experiments, primary cell stimulation, and assays measuring immune activation or cytokine secretion, where endotoxin interference with NF-κB signalling is a concern.
This protein meets > 95% as determined by reducing SDS-PAGE purity as assessed by reducing SDS-PAGE. A gel image for each lot is included on the Certificate of Analysis. Contact technical support if purity >98% is required for biophysical measurements such as crystallography, ITC, or single-particle cryo-EM.
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shelf life is 12 months from the date of receipt. Prepare small working aliquots to minimise freeze-thaw cycles of the bulk stock. Reconstituted lyophilized protein is stable for up to 3 months at −80°C in carrier protein.
BioHippo offers flexible sourcing and customisation options for qualified research institutions and commercial partners. The following add-ons may be available depending on supplier capabilities and order volume.
- Custom quantities: Bulk orders or non-standard pack sizes may be available for high-throughput or large-scale projects.
- Custom formulation: Alternative buffer compositions or lyophilisation formats may be accommodated on request.
- Biotinylated or labelled variants: Biotinylated, fluorescent, or enzymatic conjugation services may be available for select proteins.
- Extended QC documentation: Endotoxin levels, bioactivity assay data, or additional purity methods can be requested for GMP-adjacent applications.
Contact BioHippo customer support to discuss requirements. Customisation availability and lead times vary by product and supplier.
