| Field | Specification |
|---|---|
| Target | |
| Alternative names | CAIX;Carbonic Anhydrase IX;MN |
| UniProt # | |
| Accession number | |
| Species | |
| Applications | |
| Tag | |
| Expression system | |
| Expression region | |
| Molecular weight | |
| Purity | |
| Bioactivity | Measured by its esterase activity. The specific activity is > 50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
| Endotoxin level | |
| Formulation | |
| Form | Lyophilized |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
Product Description
Expression System: HEK293
Recombinant human Carbonic Anhydrase 9/CA9 is expressed in HEK293 Cells spanning Met 1-Asp 414, supplied with a C-hFc tag. Purity is 90% as determined by reducing SDS-PAGE and the product is provided as a lyophilized preparation.
Protein Specifications
| Expression Host | HEK293 Cells |
|---|---|
| Tag | C-hFc |
| Expression Region | Met 1-Asp 414 |
| Calculated MW | 67.7 kDa |
| Observed MW | 80-90 kDa |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Lead Time | 2-3 business days |
| Shelf Life | 12 months |
| Accession Number | NP_001207.2 |
| UniProt # | Q16790 |
| Alternative Names | CAIX;Carbonic Anhydrase IX;MN |
Functional Activity
Measured by its esterase activity. The specific activity is > 50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Safety & Handling
This product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.
Related Products
Explore additional recombinant proteins and matched antibodies or ELISA kits for Carbonic Anhydrase 9/CA9 research from Elabscience Biotechnology Co Ltd, or contact us for alternative tags and expression formats.
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