Recombinant Human CD44 antigen (CD44), partial (Active)

Overview

Recombinant Human CD44 antigen (CD44), partial (Active) is a recombinant protein reagent derived from Homo sapiens (Human) and produced in Mammalian cell. It is commonly used to support Cancer research by enabling binding assays, assay development and protein–protein interaction studies in controlled in vitro settings.

Key elements and design rationale

• Expressed region: 21-220aa. Region selection can focus on functional domains, improve solubility, or isolate interaction surfaces for targeted studies.
• Expression system: Mammalian cell. Expression host can influence folding and the presence/absence of post-translational modifications.
• Tag / fusion: C-terminal hFc1-tagged. Tags can support purification and detection; evaluate potential tag effects when studying sensitive interactions.
• Molecular weight (reported): 51.0 kDa. Apparent size may vary with tags, processing, and gel conditions.

When comparing results across batches or platforms, interpret signals in the context of construct design (region, tags) and expression host, especially for modification-dependent interactions.

Biological background

The gene commonly associated with this target is CD44. CD44 refers to a protein target that is studied across multiple biological contexts; annotations and nomenclature can vary by species and isoform. This product corresponds to the Homo sapiens (Human) sequence context, which can be important when comparing homologs or orthologs across model systems. For curated functional annotations, domains, and sequence features, consult primary databases (e.g., UniProt/NCBI) and the recent literature for the specific organism and isoform.

Research relevance and current trends

• Mapping pathway dependencies and signaling networks that drive tumor growth and drug resistance.
• Developing and benchmarking biomarker assays (e.g., immunoassays or binding reagents) for candidate targets.
• Characterizing protein variants, domains, or interaction partners relevant to targeted therapeutics and precision oncology.

Relevance: CDw44 (Epican) (Extracellular matrix receptor III) (ECMR-III) (GP90 lymphocyte homing/adhesion receptor) (HUTCH-I) (Heparan sulfate proteoglycan) (Hermes antigen) (Hyaluronate receptor) (PGP-1) (CD44)

Common research applications

• Assay and standard development for immunoassays or binding-based detection methods.
• Protein–protein interaction studies (e.g., receptor–ligand or complex assembly) using purified components.
• Structure–function analysis, including domain mapping or evaluation of sequence variants.

In quantitative assay development, changes in binding or activity readouts are typically interpreted relative to appropriate negative/positive controls and, where possible, orthogonal assay formats that support the same conclusion.

Notes for experimental interpretation

• Recombinant constructs may represent a defined region (domain) rather than the full-length protein; interpret results in the context of the expressed region.
• Tag or fusion elements can aid purification and detection but may influence binding surfaces or oligomerization; consider tag controls when relevant.
• Species and isoform differences can affect interaction partners and post-translational modifications; align experimental controls to the intended biological context.

• Datasheet (12927601)
• MSDS (12927601)

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