Recombinant Human Creatine Kinase MB Isoenzyme Type-2

SKU:BHP11000935
Suppliers
ProSpec-Tany TechnoGene Ltd
ProSpec-Tany TechnoGene Ltd
Details Products
Overview
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RUO recombinant Creatine Kinase MB Isoenzyme Type-2 (Human) protein for biochemical assays and mechanism studies. Supplied as a traceable protein input (Pichia pastoris; sterile-filtered solution) to support ELISA and Enzymatic assay.
Target CKMBITII
Species Human
Options selector
Catalog no. Size
cki-270-10UG 10 ug
cki-270-50UG 50 ug
cki-270-1MG 1 mg
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size (3) — 10 ug, 50 ug, 1 mg
  • Lead time: options listed as “in stock at manufacturer” typically ship in 5–7 business days; other statuses may take longer.
  • Storage: CKMBITII although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No cki-270
Alternative Names Creatine Kinase MB Isoenzyme Type-II, CKMBITII, CKMBI, CKMB.
Biological Activity The enzyme activity measured by kinetic assay at 340nm was 650 IU/mg at 37 degrees.
Expression System
  • Pichia pastoris
Form Sterile Filtered colourless liquid formulation.
Formulation Each mg of protein contains 10mM Tris-HCl, pH-6.8, 0.5mM EDTA and 0.5mM DTT, 50% (v/v) glycerol.
Product Type
  • Proteins & Peptides
  • Enzyme & Protease
Source Pichia Pastoris.
Species Human
Storage CKMBITII although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles.
Target CKMBITII

Recombinant Human Creatine Kinase MB Isoenzyme Type-2 is supplied as a recombinant protein for in vitro research use.

Background

CK-MB Type II possesses the naturally occurring carboxy-terminal amino acid lysine. This occurs during a myocardial infarct (MI or heart attack) when CK-MB Type II is released from damaged heart muscle, and the C-terminal lysine is cleaved in the blood stream, thus creating CK-MB Type I. This difference can be exploited in diagnosis of an MI.

Product format

Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.

What is the purity of Recombinant Human Creatine Kinase MB Isoenzyme Type-2 (Human)?
Purity is ≥95% as assessed by SDS-PAGE unless otherwise stated on the product datasheet or CoA.
What buffer / formulation is this protein supplied in?
Supplied as: Each mg of protein contains 10mM Tris-HCl, pH-6.8, 0.5mM EDTA and 0.5mM DTT, 50% (v/v) glycerol. Reconstitute lyophilized material in sterile ultrapure water or the recommended buffer per the datasheet prior to use.
How should Recombinant Human Creatine Kinase MB Isoenzyme Type-2 (Human) be stored?
CKMBITII although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles. Prepare single-use aliquots after reconstitution to avoid repeated freeze–thaw cycles.
What expression system was used to produce this protein?
This recombinant protein was expressed in Pichia pastoris. The system was selected to achieve high yield, correct folding, and appropriate post-translational modifications.
Is this protein biologically active?
The enzyme activity measured by kinetic assay at 340nm was 650 IU/mg at 37 degrees. Refer to the product datasheet for recommended assay conditions and controls.
Is this protein approved for clinical or in vitro diagnostic use?
No. Supplied for Research Use Only (RUO) — not intended for therapeutic applications or in vitro diagnostic procedures.
Can I request a custom size, tag variant, or formulation?
Yes. BioHippo can accommodate custom requests including alternative sizes, His/GST/Fc tag variants, bulk quantities, and custom formulations. See the Customization & Add-ons tab or email support@biohippo.com.

Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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