| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | EHK-3;EHK3;EK11;EPH Homology Kinase 3;EPH-Like Kinase 11;EPHA7;Ephrin Type-A Receptor 7;HEK11;hEK11 |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human EphB1/EPHT2 Protein — also known as EHK-3; EHK3; EK11; EPH Homology Kinase 3; EPH-Like Kinase 11; EPHA7; Ephrin Type-A Receptor 7; HEK11; hEK11 — Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synap
Research into Human EphB1/EPHT2 Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.
Product Description
Baculovirus-Insect CellsN-His-GST
Produced in Baculovirus-Insect Cells, yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
The N-His-GST enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Arg565-Ala984.
Protein Specifications
| Molecular Weight (Calculated) | 75.3 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 66 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Affinity Tag | N-His-GST |
| Expression System | Baculovirus-Insect Cells |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, 3mM DTT, pH 8.0, 10% glycerol |
| Reconstitution | Not Applicable |
| Storage Conditions | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | AAI11745.1 |
| UniProt ID | P54762 |
| Expression Region | Arg565-Ala984 |
Biological Activity
Activity ValidatedThe specific activity was determined to be 140 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH030344SpeciesHumanUniProtP54762
This protein is produced in baculovirus-infected insect cells. Baculovirus/insect expression provides intermediate post-translational modifications between bacterial and mammalian systems.
Purity: > 95% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: The specific activity was determined to be 140 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Not Applicable. Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
