Recombinant Human Erythropoietin-alpha

SKU:BHP11002235
Suppliers
ProSpec-Tany TechnoGene Ltd
ProSpec-Tany TechnoGene Ltd
Details Products
Overview
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RUO recombinant Erythropoietin-alpha (Human) protein for mechanistic studies and assay development. Supplied as a traceable protein input (CHO; >98% (SDS-PAGE) purity; lyophilized; MW 40 kDa) for reproducible assay inputs.
Target ERYTHROPOIETIN-ALPHA
Species Human
Options selector
Catalog no. Size
cyt-201-5UG 5 ug
cyt-201-50UG 50 ug
cyt-201-1MG 1 mg
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size (3) — 5 ug, 50 ug, 1 mg
  • Lead time: options listed as “in stock at manufacturer” typically ship in 5–7 business days; other statuses may take longer.
  • Storage: Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No cyt-201
Alternative Names Erythropoietin-Alpha, EPO-a, EPO-alpha, EP, MGC138142.
Biological Activity The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg.
Cellular Localization Secreted/Extracellular
Expression System
  • CHO
Form Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid.
Product Type
  • Proteins & Peptides
  • Cytokines and Growth Factors
Protein Length 166
Protein Size 40 kDa
Purity Greater than 98.0% as determined by:<br>(a) Analysis by RP-HPLC.<br>(b) Analysis by SDS-PAGE.
Solubility It is recommended to reconstitute the lyophilized EPO-alpha in sterile 18MΩ-cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Source Chinese Hamster Ovary Cells(CHO).
Species Human
Storage Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Target ERYTHROPOIETIN-ALPHA

Recombinant Human Erythropoietin-alpha is supplied as a recombinant protein for in vitro research use.

Background

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Product format

Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.

What is the purity of Recombinant Human Erythropoietin-alpha (Human)?
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. BioHippo includes a Certificate of Analysis (CoA) confirming purity per lot with every order.
What buffer / formulation is this protein supplied in?
Supplied as: Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid. Reconstitute lyophilized material in sterile ultrapure water or the recommended buffer per the datasheet prior to use.
How should Recombinant Human Erythropoietin-alpha (Human) be stored?
Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Prepare single-use aliquots after reconstitution to avoid repeated freeze–thaw cycles.
What expression system was used to produce this protein?
This recombinant protein was expressed in CHO. The system was selected to achieve high yield, correct folding, and appropriate post-translational modifications.
Is this protein biologically active?
The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg. Refer to the product datasheet for recommended assay conditions and controls.
Is this protein approved for clinical or in vitro diagnostic use?
No. Supplied for Research Use Only (RUO) — not intended for therapeutic applications or in vitro diagnostic procedures.
Can I request a custom size, tag variant, or formulation?
Yes. BioHippo can accommodate custom requests including alternative sizes, His/GST/Fc tag variants, bulk quantities, and custom formulations. See the Customization & Add-ons tab or email support@biohippo.com.

Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

1. Title :Zinc Transporters ZnT1 (Slc30a1), Zip8 (Slc39a8), and Zip10 (Slc39a10) in Mouse Red Blood Cells Are Differentially Regulated during Erythroid Development and by Dietary Zinc Deficiency 1–3 Publication :Journal of Nutrition, doi:10.3945/jn.108.093575 Vol. 138, No. 11, 2076-2083, November 2008 © 2008 American Society for Nutrition J. Nutr. 138:2076-2083, November 2008 Link : EPO a prospec publication 2. Title :Systemically delivered Erythropoietin transiently enhances adult hippocampal neurogenesis. Publication :Article first published online: 7 MAY 2007 DOI:10.1111/j.1471-4159.2007.04684.x Journal of Neurochemistry Volume 102, Issue 6, pages 1953–1965, September 2007. Link : EPO a Human prospec publication 3. Title :ZINC TRANSPORTER EXPRESSION IN MATURE RED BLOOD CELLS AND DIFFERENTIATING ERYTHROID PROGENITOR CELLS. Publication :UNIVERSITY OF FLORIDA © 2007 Moon-Suhn Ryu Link : EPO a Protein prospec publication 4. Title :Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Publication :First Published Online May 3, 2012 doi: 10.5966/sctm.2011-0048 Stem Cells Trans Med May 2012 vol. 1 no. 5 373-383 Link : Erythropoietin-Alpha prospec publication
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