| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | FGF-17;FGF17;Fibroblast Growth Factor 17 |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human FGF-17 Protein — also known as FGF-17; FGF17; Fibroblast Growth Factor 17 — Fibroblast Growth Factor 17 (FGF17) is a member of the heparin-binding growth factors family that is prominently expressed in the cerebellum and cortex. Proteins of this family possess broad mitogenic and cell survival activities and they are involved in a variety of biological processes including embryonic development cell growth; morphogenesis; tissue repair; tumor growth; and invasion. FGF17 plays an important role in the regulation of embryonic development and it acts as signaling molecule in the induction and patterning of the embryonic brain. In addition; FGF17 stimulates the proliferati
Research into Human FGF-17 Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.
Product Description
E. coli (Prokaryotic)N-His
Produced in E. coli (Prokaryotic), yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
The N-His enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Thr 23-Thr 216.
Protein Specifications
| Molecular Weight (Calculated) | 23.3 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 24 kDa |
| Purity | > 98% as determined by reducing SDS-PAGE. |
| Affinity Tag | N-His |
| Expression System | E. coli (Prokaryotic) |
| Endotoxin Level | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the prin |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | O60258 |
| UniProt ID | O60258 |
| Expression Region | Thr 23-Thr 216 |
Biological Activity
Activity ValidatedMeasure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 2 x 105 IU/mg.
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH032434SpeciesHumanUniProtO60258
This protein is produced in E. coli (prokaryotic). E. coli expression gives high yield without glycosylation — verify that your assay tolerates non-glycosylated protein.
Purity: > 98% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 0.1 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <5 ng/mL. The specific activity of recombinant human FGF-17 is > 2 x 105 IU/mg.. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
