| Field | Specification |
|---|---|
| Accession Number | |
| Alternative Names | FGF-4;FGF4;Fibroblast growth factor 4;HBGF-4;HST;HST-1;HSTF-1;HSTF1;Heparin secretory-transforming protein 1;Heparin-binding growth factor 4;KS3 ;Transforming protein KS3 |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human FGF-4/FGF4 Protein — also known as FGF-4; FGF4; Fibroblast growth factor 4; HBGF-4; HST; HST-1; HSTF-1; HSTF1; Heparin secretory-transforming protein 1; He — Fibroblast growth factor 4(FGF-4) is a heparin binding member of the FGF family. The human FGF4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature human FGF4 shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. FGF-4 is proposed t
Research into Human FGF-4/FGF4 Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.
Product Description
E. coli (Prokaryotic)
Produced in E. coli (Prokaryotic), yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
Expression region: Ser54-Leu206.
Protein Specifications
| Molecular Weight (Calculated) | 16.9 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 16 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Expression System | E. coli (Prokaryotic) |
| Endotoxin Level | < 0.01 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, 500mM NaCl, pH7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the speci |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | P08620 |
| UniProt ID | P08620 |
| Expression Region | Ser54-Leu206 |
Biological Activity
Activity ValidatedMeasured in a cell proliferation assay using MCF7 Human breast cancer cells The ED50 for this effect is 2-20 ng/ml.
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH032442SpeciesHumanUniProtP08620
This protein is produced in E. coli (prokaryotic). E. coli expression gives high yield without glycosylation — verify that your assay tolerates non-glycosylated protein.
Purity: > 95% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 0.01 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Measured in a cell proliferation assay using MCF7 Human breast cancer cells The ED50 for this effect is 2-20 ng/ml.. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
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