Recombinant Human Fibronectin Type III Domain Containing 5, Yeast

SKU:BHP11003373
Suppliers
ProSpec-Tany TechnoGene Ltd
ProSpec-Tany TechnoGene Ltd
Details Products
Overview
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RUO recombinant Fibronectin Type III Domain Containing 5 (Human) protein for mechanistic studies and assay development. Supplied as a traceable protein input (Yeast; >98% (SDS-PAGE) purity; lyophilized; MW 25 kDa) for reproducible assay inputs.
Target FRCP2
Species Human
Options selector
Catalog no. Size
pro-1663-2UG 2 ug
pro-1663-10UG 10 ug
pro-1663-1MG 1 mg
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size (3) — 2 ug, 10 ug, 1 mg
  • Lead time: options listed as “in stock at manufacturer” typically ship in 5–7 business days; other statuses may take longer.
  • Storage: Lyophilized Fibronectin Type III Domain Containing 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FNDC5 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No pro-1663
Alternative Names Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin, FRCP2, FNDC5.
Biological Activity The biological activity of recombinant human irisin was measured by the ability to induce UCP-1 expression of adipocytes. The specific activity is 10ng/ml.
Cellular Localization Secreted/Extracellular
Expression System
  • Yeast
Form Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The FNDC5 was lyophilized from 0.45 µm filtered solution in PBS.
Product Type
  • Proteins & Peptides
  • Recombinant & Natural Proteins
  • Other Protein
Protein Length 110
Protein Size 25 kDa
Purity Greater than 98.0% as determined by SDS-PAGE.
Solubility It is recommended to reconstitute the lyophilized Fibronectin Type III Domain Containing 5 in sterile 18M-cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Source Yeast.
Species Human
Storage Lyophilized Fibronectin Type III Domain Containing 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FNDC5 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Target FRCP2

Recombinant Human Fibronectin Type III Domain Containing 5, Yeast is supplied as a recombinant protein for in vitro research use.

Background

Fibronectin Type III Domain Containing 5 (Irisin) is a newly discovered hormone, secreted into circulation by muscle, which acts on white adipocytes and promotes WAT “browning”. r-Irisin can reduce body weight and cause induction of brown adipocyte in vivo, including a capacity for thermogenic energy expenditure mediated by uncoupling protein 1 (UCP-1). Irisin mature protein is comprised of 110 amino acid residues and two potential glycosylation sites. High levels of Irisin can be found in the heart whereas Very low expression is found in the colon, pancreas and spleen, if any.

Product format

Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.

What is the purity of Recombinant Human Fibronectin Type III Domain Containing 5, Yeast (Human)?
Greater than 98.0% as determined by SDS-PAGE. BioHippo includes a Certificate of Analysis (CoA) confirming purity per lot with every order.
What buffer / formulation is this protein supplied in?
Supplied as: The FNDC5 was lyophilized from 0.45 µm filtered solution in PBS. Reconstitute lyophilized material in sterile ultrapure water or the recommended buffer per the datasheet prior to use.
How should Recombinant Human Fibronectin Type III Domain Containing 5, Yeast (Human) be stored?
Lyophilized Fibronectin Type III Domain Containing 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FNDC5 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. Prepare single-use aliquots after reconstitution to avoid repeated freeze–thaw cycles.
What expression system was used to produce this protein?
This recombinant protein was expressed in Yeast. The system was selected to achieve high yield, correct folding, and appropriate post-translational modifications.
Is this protein biologically active?
The biological activity of recombinant human irisin was measured by the ability to induce UCP-1 expression of adipocytes. The specific activity is 10ng/ml. Refer to the product datasheet for recommended assay conditions and controls.
Is this protein approved for clinical or in vitro diagnostic use?
No. Supplied for Research Use Only (RUO) — not intended for therapeutic applications or in vitro diagnostic procedures.
Can I request a custom size, tag variant, or formulation?
Yes. BioHippo can accommodate custom requests including alternative sizes, His/GST/Fc tag variants, bulk quantities, and custom formulations. See the Customization & Add-ons tab or email support@biohippo.com.

Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

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