| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | CLC2;Charcot-Leyden Crystal Protein 2;Gal-14;Galectin-14;LGALS14;PPL13;Placental Protein 13-Like |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human Galectin-14/LGALS14 Protein — also known as CLC2; Charcot-Leyden Crystal Protein 2; Gal-14; Galectin-14; LGALS14; PPL13; Placental Protein 13-Like — Galectin-14 is a member of the Galectin family of carbohydrate binding proteins. The members of Galectin family contain one or two carbohydrate recognition domains, which can bind β-Galactoside. LGALS14 is expressed intracellularly in placenta and eosinophils, and is released by eosinophils following allergen stimulation. LGALS14 may be involved in the development of allergic inflammation. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
Research into Human Galectin-14/LGALS14 Protein supports investigations in cellular signaling and fundamental cell biology, with implications for cell signaling pathways.
Product Description
HEK293 Mammalian CellsC-His
Produced in HEK293 Mammalian Cells, preserving native glycosylation patterns and supporting biological activity in cell-based assays.
The C-His enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Met 1-Asp139.
Protein Specifications
| Molecular Weight (Calculated) | 17.1 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 16 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Affinity Tag | C-His |
| Expression System | HEK293 Mammalian Cells |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
| Storage Conditions | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | AAH22257.1 |
| UniProt ID | Q8TCE9 |
| Expression Region | Met 1-Asp139 |
Biological Activity
Activity ValidatedNot validated for activity
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH032472SpeciesHumanUniProtQ8TCE9
This protein is produced in HEK293 mammalian cells. Mammalian expression preserves glycosylation and folding closest to the native protein, supporting biological activity in cell assays.
Purity: > 95% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Not validated for activity. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Not Applicable. Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
