| Field | Specification |
|---|---|
| Target | |
| Alternative names | GNG1 |
| UniProt # | |
| Accession number | |
| Species | |
| Tag | |
| Expression system | |
| Expression region | |
| Molecular weight | |
| Purity | |
| Bioactivity | Not validated for activity |
| Formulation | |
| Form | Lyophilized |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
GNGT1 is a subunit of of transducin. Heterotrimeric G proteins consist of alpha, beta, and gamma subunits. They are membrane bound GTPases that are linked to 7-TM receptors. They function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. They are involved as a modulator or transducer in various transmembrane signaling systems. G proteins are bound to GDP in the 'off' state. GNGT1 is the gamma subunit of transducin. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; in addition, some Gbetagamma subunits have active functions. Gbetagamma coupled to H1 receptors can activate PLA2 and Gbetagamma coupled to M1 receptors can activate KIR channels. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Product Description
Expression System: E. coli
Recombinant human GNGT1/GNG1 is expressed in E.coli spanning Pro 2-Cys 71, supplied with a N-His tag. Purity is 90% as determined by reducing SDS-PAGE and the product is provided as a lyophilized preparation.
Protein Specifications
| Expression Host | E.coli |
|---|---|
| Tag | N-His |
| Expression Region | Pro 2-Cys 71 |
| Calculated MW | 9.9 kDa |
| Observed MW | 9.0 kDa |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin Level | Available on request |
| Formulation | Lyophilized from sterile PBS, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Lead Time | 2-3 business days |
| Shelf Life | 12 months |
| Accession Number | P63211 |
| UniProt # | P63211 |
| Alternative Names | GNG1 |
Functional Activity
This lot is supplied as a protein reagent without a validated bioactivity specification. It is suitable for binding assays, immunization, and assay development where enzymatic or receptor activity is not required.
Safety & Handling
This product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.
Related Products
Explore additional recombinant proteins and matched antibodies or ELISA kits for GNGT1/GNG1 research from Elabscience Biotechnology Co Ltd, or contact us for alternative tags and expression formats.
Can't Find What You're Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
