| Field | Specification |
|---|---|
| Target | |
| Species | |
| Expression system | |
| Source | Escherichia coli |
| Molecular weight | |
| Purity | |
| Biological activity | |
| Endotoxin level | |
| Reconstitution | |
| Formulation | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Granulocyte colony-stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ, and it plays a central role in regulating neutrophil production during steady-state granulopoiesis and in emergency responses to infection.
Product Description
Expression System: E. coli
Recombinant human G-CSF is expressed in Escherichia coli as a single non-glycosylated polypeptide chain containing 174 amino acids with an approximate molecular weight of 18.7 kDa. The protein is purified to >98% as determined by SDS-PAGE and HPLC analyses and is supplied as a sterile-filtered, white lyophilized powder.
Protein Specifications
| Source | Escherichia coli |
|---|---|
| Molecular Weight | Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
| Amino Acid Sequence | TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Endotoxin Level | Less than 1EU/mg of rHuG-CSF as determined by LAL method. |
| Formulation | Lyophilized from a 0.2mm filtered concentrated solution in 10mM sodium acetate buffer pH 4.0. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
Functional Activity
Fully biologically active when compared to standard. The ED50, as calculated by the dose-dependent proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake), is less than 0.1 ng/mL, corresponding to a specific activity of >1 × 108 IU/mg.
Safety & Handling
This product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.
Related Products
Explore additional recombinant cytokines and growth factors from Bioworld Technology Inc, or contact us for matched antibodies and ELISA kits for G-CSF research.
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