| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | FIL;UNQ;PRO;IL1F;IL1L;IL1HY;IL1RP;PSORS;IL36RN;FIL1;FIL1(DELTA);FIL1D;IL-36Ra;IL1F5;IL1HY1;IL1L1;IL1RP3;IL36RA;PSORP;PSORS14;UNQ1896;PRO4342;FIL1 Delta;IL-1 Delta;IL-1F5;IL-1HY1;IL-1L1;IL-1ra Homolog 1;IL-1-Related Protein 3;IL-1RP3;Interleukin-1 Delta;Interleukin-1 Family Member 5;Interleukin-1 HY1;Interleukin-1 Receptor Antagonist Homolog 1;Interleukin-1-Like Protein 1;Interleukin-36 Receptor Antagonist Protein |
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Scientific Background
Human IL-36 Receptor Antagonist Protein/IL-36RN/IL-1F5 — also known as FIL; UNQ; PRO; IL1F; IL1L; IL1HY; IL1RP; PSORS; IL36RN; FIL1; FIL1(DELTA); FIL1D; IL-36Ra; IL1F5; IL1HY1; IL1L1; IL1RP3; — Human Interleukin-36 Receptor Antagonist (IL-36RN) is a secreted protein which belongs to the Interleukin 1 cytokine family (IL-1 family). IL-36RN is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RN is also detected in the spleen, brain leukocyte and macrophage cell types. Increased in lesional psoriasis skin. IL-36RN is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to Interleukin 1 family member 9 (IL1F9). Dysregulated expression of novel agonistic and antagonistic IL-1 family member ligand
Research into Human IL-36 Receptor Antagonist Protein/IL-36RN/IL-1F5 supports investigations in immune regulation and inflammatory responses, with implications for cytokine biology.
Product Description
E. coli (Prokaryotic)
Produced in E. coli (Prokaryotic), yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
Expression region: Val2-Asp155.
Protein Specifications
| Molecular Weight (Calculated) | 16.8 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 17 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Expression System | E. coli (Prokaryotic) |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | Q9UBH0 |
| UniProt ID | Q9UBH0 |
| Expression Region | Val2-Asp155 |
Biological Activity
Activity ValidatedMeasured by its ability to inhibit IL-36 alpha, IL-36 beta or IL-36 gamma-induced IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 23.14 ng/ml.
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH033872SpeciesHumanUniProtQ9UBH0
This protein is produced in E. coli (prokaryotic). E. coli expression gives high yield without glycosylation — verify that your assay tolerates non-glycosylated protein.
Purity: > 95% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Measured by its ability to inhibit IL-36 alpha, IL-36 beta or IL-36 gamma-induced IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 23.14 ng/ml.. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
