| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | MGC;IL1F;IL-1F;IL18;IGIF;IL-18;IL-1g;IL1F4;GIF;Iboctadekin;IFN-gamma-inducing factor;IL-1 gamma;IL-1F4;Interferon gamma-inducing factor;Interferon-gamma-inducing factor;Interleukin 18;Interleukin-1 gamma;Interleukin-18;MGC12320;IFN gamma inducing factor;IL 1 gamma;IL 18;IL 1g;IL1 gamma;IL18 protein;IL1g;IL1gamma;ILIF4;Interferon gamma inducing factor;Interleukin 1 gamma;Interleukin 18 (interferon-gamma-inducing factor);Interleukin18 |
| Endotoxin Level | |
| Formulation | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Interleukin-18 is a secreted protein and it belongs to the IL-1 family. IL-18 is a proinflammatory cytokine and produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity.
Alternative names: MGC;IL1F;IL-1F;IL18;IGIF;IL-18;IL-1g;IL1F4;GIF;Iboctadekin;IFN-gamma-inducing factor;IL-1 gamma;IL-1F4;Interferon gamma-inducing factor;Interferon-gamma-inducing factor;Interleukin 18;Interleukin-1 gamma;Interleukin-18;MGC12320;IFN gamma inducing factor;IL 1 gamma;IL 18;IL 1g;IL1 gamma;IL18 protein;IL1g;IL1gamma;ILIF4;Interferon gamma inducing factor;Interleukin 1 gamma;Interleukin 18 (interferon-gamma-inducing factor);Interleukin18
Product Description
E. coli ExpressionThis recombinant protein is produced using a validated E. coli expression system and supplied as lyophilized powder. Reconstitute as directed on the Certificate of Analysis before use. Suitable for biochemical binding assays, ELISA standard curves, antibody validation, protein interaction studies, and related research applications.
Protein Specifications
| UniProt ID | Q14116 |
|---|---|
| Expression System | E. coli |
| Expression Region | Tyr37-Asp193 |
| Calculated MW | 18.2 kDa |
| Observed MW | 15 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0. Normally 5% - 8… |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -2… |
| Shelf Life | 12 months |
Functional Activity Data
Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is <6 ng/mL.
Safety & Handling
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
This protein was produced in E. coli expression system. The expression system determines the protein post-translational modification profile, folding characteristics, and suitability for specific applications. Mammalian (HEK293) expression is used where native-like glycosylation is required; insect cell and E. coli systems are used for high-yield non-glycosylated production.
This product is supplied as lyophilized powder. Centrifuge the vial briefly before opening, then add the recommended volume of sterile water or the specified buffer and allow to dissolve at 4°C for 30 minutes without vortexing. Prepare single-use working aliquots in 0.1% BSA to minimise freeze-thaw losses of the bulk stock.
Endotoxin is < 0.1 EU per μg of the protein as determined by the LAL method., determined by the LAL chromogenic method. The <1.0 EU/µg specification ensures suitability for cell culture experiments, primary cell stimulation, and assays measuring immune activation or cytokine secretion, where endotoxin interference with NF-κB signalling is a concern.
This protein meets > 95% as determined by reducing SDS-PAGE purity as assessed by reducing SDS-PAGE. A gel image for each lot is included on the Certificate of Analysis. Contact technical support if purity >98% is required for biophysical measurements such as crystallography, ITC, or single-particle cryo-EM.
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shelf life is 12 months from the date of receipt. Prepare small working aliquots to minimise freeze-thaw cycles of the bulk stock. Reconstituted lyophilized protein is stable for up to 3 months at −80°C in carrier protein.
BioHippo offers flexible sourcing and customisation options for qualified research institutions and commercial partners. The following add-ons may be available depending on supplier capabilities and order volume.
- Custom quantities: Bulk orders or non-standard pack sizes may be available for high-throughput or large-scale projects.
- Custom formulation: Alternative buffer compositions or lyophilisation formats may be accommodated on request.
- Biotinylated or labelled variants: Biotinylated, fluorescent, or enzymatic conjugation services may be available for select proteins.
- Extended QC documentation: Endotoxin levels, bioactivity assay data, or additional purity methods can be requested for GMP-adjacent applications.
Contact BioHippo customer support to discuss requirements. Customisation availability and lead times vary by product and supplier.
