| Field | Specification |
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| Accession Number | |
| Alternative Names | KLK8;Kallikrein-8;NP;NRPN;Neuropsin;Ovasin;PRSS19;Serine Protease 19;Serine Protease TADG-14;TADG14;Tumor-Associated Differentially Expressed Gene 14 Protein;hK8 |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
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| UniProt # |
Scientific Background
Human KLK-8/Kallikrein-8 Protein — also known as KLK8; Kallikrein-8; NP; NRPN; Neuropsin; Ovasin; PRSS19; Serine Protease 19; Serine Protease TADG-14; TADG14; Tumor-Asso — Kallikrein-8, also known as Neuropsin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein and KLK8, is a secreted protein which belongs to thepeptidase S1 family and Kallikrein subfamily. It is a serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Kallikrein-8 / KLK8 plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway. It regulates Schaffer-collateral long-term potentiation in the hip
Research into Human KLK-8/Kallikrein-8 Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.
Product Description
HEK293 Mammalian CellsC-His
Produced in HEK293 Mammalian Cells, preserving native glycosylation patterns and supporting biological activity in cell-based assays.
The C-His enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Met 1-Gly 260.
Protein Specifications
| Molecular Weight (Calculated) | 26.4 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 36 kDa |
| Purity | > 98% as determined by reducing SDS-PAGE. |
| Affinity Tag | C-His |
| Expression System | HEK293 Mammalian Cells |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the prin |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | O60259-1 |
| UniProt ID | O60259 |
| Expression Region | Met 1-Gly 260 |
Biological Activity
Activity ValidatedMeasured by its ability to cleave the fluorogenic peptide substrate BocVPRAMC. The specific activity is > 400pmoles/min/μg
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH030307SpeciesHumanUniProtO60259
This protein is produced in HEK293 mammalian cells. Mammalian expression preserves glycosylation and folding closest to the native protein, supporting biological activity in cell assays.
Purity: > 98% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Measured by its ability to cleave the fluorogenic peptide substrate BocVPRAMC. The specific activity is > 400pmoles/min/μg. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
