| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | CDF;D Factor;DIA;Differentiation-Stimulating Factor;Emfilermin;HILDA;LIF;Leukemia Inhibitory Factor;MLPLI;Melanoma-Derived LPL Inhibitor |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human LIF Protein — also known as CDF; D Factor; DIA; Differentiation-Stimulating Factor; Emfilermin; HILDA; LIF; Leukemia Inhibitory Factor; MLPLI; Melan — Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation; control of stem cell pluripotency; bone and fat metabolism; mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human
Research into Human LIF Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.
Product Description
E. coli (Prokaryotic)N-His
Produced in E. coli (Prokaryotic), yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
The N-His enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Ser23-Phe202.
Protein Specifications
| Molecular Weight (Calculated) | 20.5 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 18 kDa |
| Purity | > 98% as determined by reducing SDS-PAGE. |
| Affinity Tag | N-His |
| Expression System | E. coli (Prokaryotic) |
| Endotoxin Level | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the pri |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | P15018 |
| UniProt ID | P15018 |
| Expression Region | Ser23-Phe202 |
Biological Activity
Activity ValidatedMeasure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.2 ng/mL.
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH032694SpeciesHumanUniProtP15018
This protein is produced in E. coli (prokaryotic). E. coli expression gives high yield without glycosylation — verify that your assay tolerates non-glycosylated protein.
Purity: > 98% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 0.1 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.2 ng/mL.. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
