| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | MAPKAP-K5;MK-5;MK5;PRAK |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human MAPKAPK5 Protein — also known as MAPKAP-K5; MK-5; MK5; PRAK — MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by
Research into Human MAPKAPK5 Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.
Product Description
Baculovirus-Insect CellsN-His-GST
Produced in Baculovirus-Insect Cells, yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
The N-His-GST enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Met 1-Gln 471.
Protein Specifications
| Molecular Weight (Calculated) | 82.0 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 75 kDa |
| Purity | > 76% as determined by reducing SDS-PAGE. |
| Affinity Tag | N-His-GST |
| Expression System | Baculovirus-Insect Cells |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol |
| Reconstitution | Not Applicable |
| Storage Conditions | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | NP_003659.2 |
| UniProt ID | Q8IW41 |
| Expression Region | Met 1-Gln 471 |
Biological Activity
Activity ValidatedNot validated for activity
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH030328SpeciesHumanUniProtQ8IW41
This protein is produced in baculovirus-infected insect cells. Baculovirus/insect expression provides intermediate post-translational modifications between bacterial and mammalian systems.
Purity: > 76% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Not validated for activity. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Not Applicable. Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
