| Field | Specification |
|---|---|
| Target | |
| Alternative names | CLG;Fibroblast Collagenase;Interstitial Collagenase;MMP-1;MMP1;Matrix Metalloproteinase-1 |
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| Accession number | |
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| Molecular weight | |
| Purity | |
| Bioactivity | Measured by its ability to cleave the fluorogenic peptide substrate, McaPLGL-Dpa-AR-NH2, R&D System, Cat#ES010.The specific activity is > 400 pmoles/min/μg(Activation description: The proenzyme needs to be activated by APMA for an activated form) |
| Endotoxin level | |
| Formulation | |
| Form | Lyophilized |
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Scientific Background
MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. Matrix metalloproteases, also called matrixins, are zinc-dependent endopeptidases that are the major proteases involved in ECM degradation. MMPs are capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. MMP activity is regulated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMPs play a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Tumour metastasis is a multistep process involving the dessemination of tumor cells from the primary tumor to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves or by surrounding stromal cells stimulated by the nearby tumor. MMP-1, -2, -3, -7, -9, -13 and -14 all have elevated expression in primary tumors and/or metastases. MMP-1 cleaves collagens of types I, II, and III at one site in the helical domain. It also cleaves collagens of types VII and X. In case of HIV infection, MMP1 interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Product Description
Expression System: HEK293
Recombinant human MMP1 is expressed in HEK293 Cells spanning Met 1-Asn 469, supplied with a C-His tag. Purity is 96% as determined by reducing SDS-PAGE and the product is provided as a lyophilized preparation.
Protein Specifications
| Expression Host | HEK293 Cells |
|---|---|
| Tag | C-His |
| Expression Region | Met 1-Asn 469 |
| Calculated MW | 54.8 kDa |
| Observed MW | 50-55 kDa |
| Purity | > 96% as determined by reducing SDS-PAGE. |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile 25mM MES, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 5.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Lead Time | 2-3 business days |
| Shelf Life | 12 months |
| Accession Number | NP_002412.1 |
| UniProt # | P03956 |
| Alternative Names | CLG;Fibroblast Collagenase;Interstitial Collagenase;MMP-1;MMP1;Matrix Metalloproteinase-1 |
Functional Activity
Measured by its ability to cleave the fluorogenic peptide substrate, McaPLGL-Dpa-AR-NH2, R&D System, Cat#ES010.The specific activity is > 400 pmoles/min/μg(Activation description: The proenzyme needs to be activated by APMA for an activated form)
Safety & Handling
This product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.
Related Products
Explore additional recombinant proteins and matched antibodies or ELISA kits for MMP1 research from Elabscience Biotechnology Co Ltd, or contact us for alternative tags and expression formats.
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